规格

价格

库存

数量

10 mg

¥ 119

现货

25 mg

¥ 243

现货

50 mg

¥ 367

现货

100 mg

¥ 546

现货

1 mL x 10 mM (in DMSO)

¥ 195

现货

生物活性

产品描述	3,6-Dihydroxyflavone suppresses the epithelial-mesenchymal transition in breast cancer cells by inhibiting the Notch signaling pathway.
体外活性	3,6-Dihydroxyflavone（3,6-DHF）能有效抑制体外和体内乳腺癌（BC）细胞的上皮间质转化（EMT）。研究表明，3,6-DHF有效抑制乳腺癌干细胞（BCSCs）的形成和增殖，从而降低了小鼠NOD/SCID中肿瘤细胞的肿瘤形成能力。体内光学成像显示，3,6-DHF治疗抑制了乳腺癌细胞在体内的肺转移。进一步研究指出，3,6-DHF通过下调Notch1、NICD、Hes-1和c-Myc，减少NICD-CSL-MAML功能性转录单元的形成，从而导致乳腺癌细胞中Notch信号通路的失活。Notch1的过度表达或miR-34a的抑制显著降低了3,6-DHF对EMT、CSCs以及乳腺癌细胞迁移和侵袭的抑制作用。
细胞实验	Cells were cultured in six-well plates until they reached 100% confluence. A vertical or horizontal wound was gently created in monolayers using a 20 μl sterile pipette tip. The cells were then washed for 3 times with growth medium to remove the detached cells, and the medium was added with fresh medium treated with 3,6-DHF. Images were captured using an inverted microscope and camera at designed times to assess the inhibition of wound closure[1].
别名	3,6-二羟基黄酮,97%

化学信息

分子量	254.24
分子式	C₁₅H₁₀O₄
CAS No.	108238-41-1
Smiles	Oc1ccc2oc(-c3ccccc3)c(O)c(=O)c2c1
密度	1.472g/cm3

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

实际储存温度请以COA为准

溶解度信息

DMSO: 250 mg/mL (983.32 mM), Sonication is recommended.

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.87 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.9333 mL	19.6665 mL	39.3329 mL	196.6646 mL
5 mM	0.7867 mL	3.9333 mL	7.8666 mL	39.3329 mL
10 mM	0.3933 mL	1.9666 mL	3.9333 mL	19.6665 mL
20 mM	0.1967 mL	0.9833 mL	1.9666 mL	9.8332 mL
50 mM	0.0787 mL	0.3933 mL	0.7867 mL	3.9333 mL
100 mM	0.0393 mL	0.1967 mL	0.3933 mL	1.9666 mL

该溶液配制表仅适用于固体产品。对于液体产品，请根据标明的浓度或密度计算稀释方案。

3,6-Dihydroxyflavone

3,6-Dihydroxyflavone

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词

3,6-Dihydroxyflavone

3,6-Dihydroxyflavone