规格

价格

库存

数量

1 mg

¥ 872

现货

5 mg

¥ 2,530

现货

10 mg

¥ 3,690

现货

25 mg

¥ 5,890

现货

50 mg

¥ 7,930

现货

100 mg

¥ 10,700

现货

1 mL x 10 mM (in DMSO)

¥ 3,250

现货

生物活性

产品描述	Z-DEVD-FMK (Caspase-3 Inhibitor) is a Caspase-3 inhibitor (IC50=18 μM), featuring selectivity, irreversibility and cell permeability. Z-DEVD-FMK can inhibit caspase activation induced apoptosis.
靶点活性	Caspase-3:18 μM.
体外活性	方法：v-K-ras转化的正常大鼠肾脏细胞 KNRK 用 SCH56582 (20 µM) 和 Z-DEVD-FMK (20-50 µM) 处理 24 h，使用 trypan blue 检测细胞死亡。结果：添加 50 µM Z-DEVD-fmk 导致细胞凋亡抑制 >70%。[1] 方法：人多发性骨髓瘤细胞 KM3 用 betulinic acid (15 µg/mL) 和 Z-DEVD-FMK (50 mol/L) 处理 24 h，使用 Western Blot 检测靶点蛋白表达水平。结果：Z-DEVD-VMK 减轻了 betulinic acid 诱导的 caspase 3 的激活。Z-DEVD-FMK 显著阻断 PARP 的切割。[2]
体内活性	方法：为测试体内活性，将 Z-DEVD-FMK (1.8 mg/kg in ethanol and freshly diluted in PBS containing 2% Tween-80) 腹腔注射给 C57BL/6 小鼠，30 min 后注射 CPT-11 (350 mg/kg)。Z-DEVD-FMK 继续给药三天，每天一次。结果：Caspase-3 抑制剂 Z-DEVD-FMK 减弱 PT-11 诱导的 GATA6 缺失小鼠腹腔巨噬细胞。[3]
激酶实验	Caspase activity assay : Caspase-3 and caspase-9 activities are measured using fluorescent-based substrate. After treatment, the cells are resuspended in lysis buffer (50 mM Tris HCl, 1 mM EDTA, and 10 mM EGTA) containing 10 mM digitonin for 20 min at 37°C. Supernatants are treated with either of the fluorogenic substrates Ac-DEVD-AFC for caspase-3 or Ac-LEHD-AFC for caspase-9 for 1 h at 37°C and fluorescence is measured at excitation at 400 nm and emission at 505 nm using a Gemini XS fluorescence plate reade
细胞实验	N27 cells are incubated with 100 μM 6-OHDA for 24 h or 300 μM MPP+ for 36 h in the presence or absence of 50 μM Z-DEVD-FMK and cell death is determined by MTT (3-(4,5-dimethylthiazol-3-yl)-2,5-diphenyl tetrazolium bromide) assay, which is widely used to assess cell viability. After treatment, the cells are incubated in serum-free medium containing 0.25 mg/ml MTT for 3 h at 37°C. Formation of formazan from tetrazolium is measured at 570 nm with a reference wavelength at 630 nm using a SpectraMax microplate reader.(Only for Reference)
别名	Caspase-3 Inhibitor

化学信息

分子量	668.66
分子式	C₃₀H₄₁FN₄O₁₂
CAS No.	210344-95-9
Smiles	COC(=O)CC[C@H](NC(=O)[C@H](CC(=O)OC)NC(=O)OCc1ccccc1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(=O)OC)C(=O)CF
密度	1.260 g/cm3 (Predicted)
Sequence	Cbz-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-CH2F
Sequence Short	XXVX

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

实际储存温度请以COA为准

溶解度信息

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 252.5 mg/mL (377.62 mM), Sonication is recommended.

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (7.48 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.4955 mL	7.4776 mL	14.9553 mL	74.7764 mL
5 mM	0.2991 mL	1.4955 mL	2.9911 mL	14.9553 mL
10 mM	0.1496 mL	0.7478 mL	1.4955 mL	7.4776 mL
20 mM	0.0748 mL	0.3739 mL	0.7478 mL	3.7388 mL
50 mM	0.0299 mL	0.1496 mL	0.2991 mL	1.4955 mL
100 mM	0.0150 mL	0.0748 mL	0.1496 mL	0.7478 mL

该溶液配制表仅适用于固体产品。对于液体产品，请根据标明的浓度或密度计算稀释方案。

Z-DEVD-FMK

Z-DEVD-FMK

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词

Z-DEVD-FMK

Z-DEVD-FMK

Z-DEVD-FMK 相关产品

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词