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顶部生物活性储存和溶解信息计算器

Anti-Caspase-3 Polyclonal Antibody

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产品编号 TMAB-00285

别名 Yama, SCA-1, CPP32B, CPP32, apopain

Anti-Caspase-3 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Caspase-3。Anti-Caspase-3 Polyclonal Antibody 可用于 ELISA,WB。

Anti-Caspase-3 Polyclonal Antibody

Anti-Caspase-3 Polyclonal Antibody

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产品编号 TMAB-00285 别名 Yama, SCA-1, CPP32B, CPP32, apopain

Anti-Caspase-3 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Caspase-3。Anti-Caspase-3 Polyclonal Antibody 可用于 ELISA,WB。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,975
5日内发货
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生物活性
产品描述
Anti-Caspase-3 Polyclonal Antibody is a Rabbit antibody targeting Caspase-3. Anti-Caspase-3 Polyclonal Antibody can be used in ELISA,WB.
别名Yama, SCA-1, CPP32B, CPP32, apopain
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Sample:
Lane 1: Raji (Human) Cell Lysate at 30 μg
Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 3: Lung (Mouse) Lysate at 40 μg
Lane 4: Lung (Rat) Lysate at 40 μg
Primary: Anti-Caspase-3 (TMAB-00285) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 37 kDa
2. Sample:
Lane 1: Spleen (Mouse) Lysate at 40 μg
Lane 2: Lung (Mouse) Lysate at 40 μg
Lane 3: LympHnode (Mouse) Lysate at 40 μg
Lane 4: Cerebrum (Mouse) Lysate at 40 μg
Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 6: Spleen (Rat) Lysate at 40 μg
Lane 7: Lung (Rat) Lysate at 40 μg
Lane 8: LympHnode (Rat) Lysate at 40 μg
Lane 9: Cerebrum (Rat) Lysate at 40 μg
Lane 10: Hela (Human) Cell Lysate at 30 μg
Primary: Anti-Caspase-3 (TMAB-00285) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 37 kDa
verifiedActivityverifiedActivity
应用ELISAWB
推荐剂量
WB: 1:500-2000; ELISA: 1:5000-10000
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cytoplasm.
组织特异性Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
抗原信息
免疫原
KLH conjugated synthetic peptide: human caspase-3 p17 subunit
抗原种属
Human
基因名称
CASP3
基因ID
836
蛋白名称
Caspase-3
Uniprot ID
P42574
研究领域
Caspases,Metabolism,Caspases,Caspases,Other proteases,Apoptosis
功能
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
化学信息
分子量Theoretical: 17/32 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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