Powder: -20°C for 3 years | In solvent: -80°C for 1 year
SY-LB-57 是骨形态发生蛋白 (BMP) 受体信号传导的高效激动剂。SY-LB-57可用于骨折和肺动脉高压等病症的研究。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 642 | 5日内发货 | ||
5 mg | ¥ 1,080 | 5日内发货 | ||
10 mg | ¥ 1,980 | 5日内发货 |
产品描述 | SY-LB-57 is a highly effective agonist of bone morphogenetic protein (BMP) receptor signaling. SY-LB-57 can be used in studies of conditions such as bone fracture and pulmonary arterial hypertension [1]. |
体外活性 | SY-LB-57 (0.01-1000 μM, 24 h) can markedly induce cell proliferation at concentrations below 10 μM with an IC 50 value of 807.93 μM in C2C12 cells [1]. SY-LB-57 (0.01-10 μM, 15-30 min) increases the Smad protein phosphorylation and promotes p-Smad nuclear translocation, activates the PI3K/Akt pathway, and induces cytoplasmic localization of p-Akt [1]. SY-LB-57 (0.01-10 μM, 24 h) can induce cell cycle shift towards S and G2/M phases [1]. Cell Viability Assay [1] Cell Line: C2C12 cells Concentration: 0.01-1000 μM Incubation Time: 24 hours Result: Concentrations of 0.01–100 µM SY-LB-57 increased cell viability in C2C12 cells significantly compared with control by 280%, 290%, 200%, 150%, and 50% at 0.01 μM, 0.1 μM, 1 μM, 10 μM, and 100 μM, respectively. Western Blot Analysis [1] Cell Line: C2C12 cells Concentration: 0.01-10 μM Incubation Time: 15min or 30 min Result: strongly increased phosphorylation of Smad protein, 50% to 257% higher compared to control after 30 min. Showed a significant, robust increase in p-Akt expression levels over the control by 667-1081% after 15 min. Cell Cycle Analysis [1] Cell Line: C2C12 cells Concentration: 0.01-10 μM Incubation Time: 24 hours Result: Shifted the cell cycle in C2C12 cells toward proliferation causing a significant decline in the population of cells in G0/G1 phase and a sharp increase in the percentage of cells present in proliferative phases of the cell cycle (S and G2/M). |
分子量 | 263.29 |
分子式 | C16H13N3O |
CAS No. | 2253719-35-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
SY-LB-57 2253719-35-4 Inhibitor inhibitor inhibit