规格

价格

库存

数量

1 mg

¥ 142

现货

5 mg

¥ 293

现货

10 mg

¥ 483

现货

25 mg

¥ 967

现货

50 mg

¥ 1,480

现货

100 mg

¥ 2,250

现货

200 mg

¥ 3,350

现货

1 mL x 10 mM (in DMSO)

¥ 329

现货

生物活性

产品描述	ML335 is a selective activator of TREK-1 and TREK-2.
体外活性	Xenopus oocyte two-electrode voltage-clamp measurements show that ML335 and ML402 activate K2P2.1 and K2P10.1 but not K2P4.1 (14.3±2.7?μM, K2P2.1-ML335; 13.7±7.0?μM, K2P2.1-ML402; 5.2±0.5?μM, K2P10.1-ML335; and 5.9±1.6?μM, K2P10.1-ML402). Swapping the Lys271 equivalent between K2P2.1 and K2P4.1 results in a clear phenotype reversal for ML335 and M402 activation. ML335 and ML402 activate K2P2.1 in HEK293 cells similar to their effects in Xenopus oocytes (5.2±0.8?μM and 5.9±1.6?μM for ML335 and ML402, respectively (n≥3))
细胞实验	Mouse K2P2.1, human K2P4.1, and mutants are expressed from a previously described pIRES2-EGFP vector in HEK293T cells (ATTC). 70% confluent cells are transfected (in 35-mm diameter wells) with LipofectAMINE 2000 for 6?h, and plated onto coverslips coated with Matrigel. Effects of ML335, ML402 and arachidonic acid on K2P2.1 current at 0?mV are measured by whole-cell patch-clamp experiments 24?h after transfection. Acquisition and analysis are performed using pCLAMP9 and an Axopatch 200B amplifier. Pipette resistance ranges from 1 to 1.5?MΩ. Pipette solution contains the following: 145?mM KCl, 3?mM MgCl2, 5?mM EGTA and 20?mM HEPES (pH 7.2 with KOH). Bath solution contains the following: 145?mM NaCl, 5?mM KCl, 1?mM CaCl2, 3?mM MgCl2 and 20?mM HEPES (pH 7.4 with NaOH). K2P2.1 currents are elicited by a 1?s ramp from -100 to +50?mV from a -80?mV holding potential. After stabilization of the basal current, ML335 and ML402 are perfused at 200?mL per hour until potentiation is stably reached[1].

化学信息

分子量	373.25
分子式	C₁₅H₁₄Cl₂N₂O₃S
CAS No.	825658-06-8
Smiles	CS(=O)(=O)Nc1ccc(cc1)C(=O)NCc1ccc(Cl)cc1Cl
密度	no data available

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

实际储存温度请以COA为准

溶解度信息

DMSO: 45 mg/mL (120.56 mM), Sonication is recommended.

体内实验配方

10% DMSO+90% Corn Oil: 2 mg/mL (5.36 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.6792 mL	13.3958 mL	26.7917 mL	133.9585 mL
5 mM	0.5358 mL	2.6792 mL	5.3583 mL	26.7917 mL
10 mM	0.2679 mL	1.3396 mL	2.6792 mL	13.3958 mL
20 mM	0.1340 mL	0.6698 mL	1.3396 mL	6.6979 mL
50 mM	0.0536 mL	0.2679 mL	0.5358 mL	2.6792 mL
100 mM	0.0268 mL	0.1340 mL	0.2679 mL	1.3396 mL

该溶液配制表仅适用于固体产品。对于液体产品，请根据标明的浓度或密度计算稀释方案。

ML335

ML335

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词

ML335

ML335

ML335 相关产品

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词