规格

价格

库存

数量

25 mg

¥ 198

现货

50 mg

¥ 289

现货

100 mg

¥ 413

现货

500 mg

¥ 793

现货

1 g

¥ 1,190

现货

1 mL x 10 mM (in DMSO)

¥ 223

现货

生物活性

产品描述	Amphotericin B (NSC-527017) is a polyene antifungal agent with broad-spectrum activity against many fungal species. Amphotericin B irreversibly binds to ergosterol and disrupts the integrity of cell membranes, resulting in antifungal activity.
靶点活性	Trichophyton mentagrophytes:0.02 µg/mL, Leishmania donovani:0.06 µg/mL, Candida species:2-8 µg/mL (MIC50), Adherence to plastic:5.1 mg/L (PMNs)
体外活性	方法：人正常结肠上皮细胞 CCD 841 CoTr 和人结直肠癌细胞 HT-29 用含 Amphotericin B (0.05-25 µg/mL) 处理 24 h，使用 Neutral Red method 检测细胞活力。结果：较高浓度的 Amphotericin B 对 CCD 841 CoTr 和 HT-29 细胞有毒，IC50 值分别为 8.7 µg/mL 和 21.2 µg/mL。[1] 方法：Candida albicans 用 Amphotericin B (100 µM) 处理 4-16 min，使用 FLIM technique 进行成像。结果：Amphotericin B 优先与细胞壁结合，而不会有效地穿过覆盖细胞膜的屏障。Amphotericin B 在出芽阶段可以更容易地通过年轻细胞的细胞壁屏障。[2]
体内活性	方法：为检测体内抗真菌活性，将 Amphotericin B (0.25-4 mg/kg) 单次腹腔注射给 C. albicans K-1 感染的 ICR/Swiss 小鼠。结果：与治疗开始时的菌落数相比，只有最高单次剂量的 Amphotericin B 治疗才能显著减少菌落数。[3]
激酶实验	THP-1 and HEK293 cells are transiently transfected using DEAE-dextran and Polyfect reagent, respectively. Plasmids transfected contain genes coding for the NF-κB-dependent pELAM-luc luciferase reporter, TLR2, TLR4, CD14, and MD2. Cells (5×105 THP-1 or 1×105 HEK293) are added to 12-well plates, washed after 18 h, and stimulated for 5 h. Cells are then lysed with reporter lysis buffer as directed, and lysates are analyzed for luminescence using Promega luciferase substrate and a Monolight 3010 luminometer.
细胞实验	Amphotericin B is dissolved in DMSO. The kinetics of cell death induced by AmB against Leishmania promastigotes is followed by using fluorometry with the DNA-binding compound ethidium bromide (EB). Fluorescence measurements are performed on a SPEX Fluorolog II spectrophotometer at 365-580 nm excitation-emission wavelengths. Promastigotes at a final concentration of 25×106 cells/mL are incubated for 5 min with gentle stirring in the fluorescence cuvette with 2 mL of different buffered solutions but always containing 10 mM glucose and EB (50 mM). After signal stabilization is achieved, AmB is added and dissolved in dimethylsulfoxide. Maximal EB incorporation is always obtained by adding digitonin (50 mg/mL). All solutions used are buffered with 75 mM TRIS (pH 4 7.6) and contain 150 mM NaCl (BNa+), 150 mM KCl (BK+), 150 mM choline chloride, and 100 mM sucrose, 100 mM NaCl. The osmolarity of all solutions is always adjusted to 390±5 mOsm using an advanced instrument SW2 osmometer.
别名	两性霉素B, NSC 527017

化学信息

分子量	924.08
分子式	C₄₇H₇₃NO₁₇
CAS No.	1397-89-3
Smiles	[H][C@]12C[C@@H](O[C@@H]3O[C@H](C)[C@@H](O)[C@H](N)[C@@H]3O)\C=C\C=C\C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]1C(O)=O)O2
密度	1.34 g/cm3

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

实际储存温度请以COA为准

溶解度信息

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 51 mg/mL (55.19 mM), Sonication is recommended.

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (5.41 mM), Suspension.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.0822 mL	5.4108 mL	10.8216 mL	54.1079 mL
5 mM	0.2164 mL	1.0822 mL	2.1643 mL	10.8216 mL
10 mM	0.1082 mL	0.5411 mL	1.0822 mL	5.4108 mL
20 mM	0.0541 mL	0.2705 mL	0.5411 mL	2.7054 mL
50 mM	0.0216 mL	0.1082 mL	0.2164 mL	1.0822 mL

该溶液配制表仅适用于固体产品。对于液体产品，请根据标明的浓度或密度计算稀释方案。

Amphotericin B

Amphotericin B

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词

Amphotericin B

Amphotericin B

Amphotericin B 相关产品

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词