store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Amphotericin B (NSC-527017) 是一种多烯类抗真菌剂,对许多真菌物种具有广谱活性。Amphotericin B 不可逆地结合麦角甾醇,破坏细胞膜完整性,从而起到抗真菌活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 mg | ¥ 298 | 待询 | ||
100 mg | ¥ 418 | 待询 | ||
500 mg | ¥ 823 | 待询 | ||
1 mL * 10 mM (in DMSO) | ¥ 460 | 现货 |
产品描述 | Amphotericin B (NSC-527017) is a polyene antifungal agent with broad-spectrum activity against many fungal species. Amphotericin B irreversibly binds to ergosterol and disrupts the integrity of cell membranes, resulting in antifungal activity. |
体外活性 |
方法:人正常结肠上皮细胞 CCD 841 CoTr 和人结直肠癌细胞 HT-29 用含 Amphotericin B (0.05-25 µg/mL) 处理 24 h,使用 Neutral Red method 检测细胞活力。 结果:较高浓度的 Amphotericin B 对 CCD 841 CoTr 和 HT-29 细胞有毒,IC50 值分别为 8.7 µg/mL 和 21.2 µg/mL。[1] 方法:Candida albicans 用 Amphotericin B (100 µM) 处理 4-16 min,使用 FLIM technique 进行成像。 结果:Amphotericin B 优先与细胞壁结合,而不会有效地穿过覆盖细胞膜的屏障。Amphotericin B 在出芽阶段可以更容易地通过年轻细胞的细胞壁屏障。[2] |
体内活性 |
方法:为检测体内抗真菌活性,将 Amphotericin B (0.25-4 mg/kg) 单次腹腔注射给 C. albicans K-1 感染的 ICR/Swiss 小鼠。 结果:与治疗开始时的菌落数相比,只有最高单次剂量的 Amphotericin B 治疗才能显著减少菌落数。[3] |
激酶实验 | THP-1 and HEK293 cells are transiently transfected using DEAE-dextran and Polyfect reagent, respectively. Plasmids transfected contain genes coding for the NF-κB-dependent pELAM-luc luciferase reporter, TLR2, TLR4, CD14, and MD2. Cells (5×105 THP-1 or 1×105 HEK293) are added to 12-well plates, washed after 18 h, and stimulated for 5 h. Cells are then lysed with reporter lysis buffer as directed, and lysates are analyzed for luminescence using Promega luciferase substrate and a Monolight 3010 luminometer. |
细胞实验 | Amphotericin B is dissolved in DMSO. The kinetics of cell death induced by AmB against Leishmania promastigotes is followed by using fluorometry with the DNA-binding compound ethidium bromide (EB). Fluorescence measurements are performed on a SPEX Fluorolog II spectrophotometer at 365-580 nm excitation-emission wavelengths. Promastigotes at a final concentration of 25×106 cells/mL are incubated for 5 min with gentle stirring in the fluorescence cuvette with 2 mL of different buffered solutions but always containing 10 mM glucose and EB (50 mM). After signal stabilization is achieved, AmB is added and dissolved in dimethylsulfoxide. Maximal EB incorporation is always obtained by adding digitonin (50 mg/mL). All solutions used are buffered with 75 mM TRIS (pH 4 7.6) and contain 150 mM NaCl (BNa+), 150 mM KCl (BK+), 150 mM choline chloride, and 100 mM sucrose, 100 mM NaCl. The osmolarity of all solutions is always adjusted to 390±5 mOsm using an advanced instrument SW2 osmometer. |
别名 | NSC 527017, 两性霉素B |
分子量 | 924.08 |
分子式 | C47H73NO17 |
CAS No. | 1397-89-3 |
store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
5% DMSO+95% Saline: 1.05 mg/mL (1.14 mM, suspension)
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 50 mg/mL (54.11 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
5% DMSO+95% Saline / DMSO | 1 mM | 1.0822 mL | 5.4108 mL | 10.8216 mL | 27.0539 mL |
DMSO | 5 mM | 0.2164 mL | 1.0822 mL | 2.1643 mL | 5.4108 mL |
10 mM | 0.1082 mL | 0.5411 mL | 1.0822 mL | 2.7054 mL | |
20 mM | 0.0541 mL | 0.2705 mL | 0.5411 mL | 1.3527 mL | |
50 mM | 0.0216 mL | 0.1082 mL | 0.2164 mL | 0.5411 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Amphotericin B 1397-89-3 Microbiology/Virology Antibiotic Antifungal Fungal NSC527017 NSC-527017 Inhibitor NSC 527017 两性霉素B inhibit inhibitor