购物车

您的购物车当前为空

25-Hydroxycholesterol

Name: 25-Hydroxycholesterol
Brand: TargetMol
SKU: T4717
Price: 423 CNY
Availability: InStock
Rating: 5 (10 reviews)

一键复制产品信息

很棒

纯度: 99.89%

货号 T4717Cas号 2140-46-7

别名 25-羟基胆甾醇, 25-羟基胆固醇

25-Hydroxycholesterol 是胆固醇的代谢产物，由巨噬细胞响应 Toll 样受体激活而分泌。它有效抑制 B 细胞产生 IgA，EC50约为 65 nM。

为众多的药物研发团队赋能，

让新药发现更简单！

25-Hydroxycholesterol

一键复制产品信息

很棒

纯度: 99.89%

货号 T4717 别名 25-羟基胆甾醇, 25-羟基胆固醇Cas号 2140-46-7

25-Hydroxycholesterol 是胆固醇的代谢产物，由巨噬细胞响应 Toll 样受体激活而分泌。它有效抑制 B 细胞产生 IgA，EC50约为 65 nM。

规格	价格	库存
5 mg	¥ 423	现货
10 mg	¥ 581	现货
25 mg	¥ 1,149	现货
50 mg	¥ 1,995	现货
100 mg	¥ 2,995	现货

库存状态实时更新，以官网显示为准，现货产品可直接加购物车下单

大包装 & 定制

加入购物车

TargetMol的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。

实验操作小课堂

常见问题解答

选择批次:

纯度: 99.89%

联系我们获取更多批次信息

资源下载:

COA HNMR LCMS 产品操作手册

产品介绍

25-Hydroxycholesterol AI Summary

25-Hydroxycholesterol exhibits a multifaceted pharmacological profile with significant bioactivities across various assays and targets. It activates the human nuclear oxysterol receptor liver X receptor-alpha (LXR-alpha) with an EC50 of 1160.0 nM and a relative efficacy of 0.35. As an HMG-CoA reductase inhibitor, it shows a 70.0% inhibition of transcription in HepG2 cells and an IC50 of 300.0 nM, suggesting potential for managing cholesterol metabolism. It also demonstrates agonist activity at the hedgehog receptor (Hh) with an EC50 of 1000.0 nM and induces RORgamma LBD coactivator recruitment with an EC50 of 20.0 nM. 25-Hydroxycholesterol effectively inhibits SREBP2 activation in CHO-K1 cells and shows cytotoxicity against human HepG2 cells (>70.0% at 48 hours as assessed by MTT assay). Additionally, it exhibits binding affinity to NPC1L1 mutants expressed in HEK293 cells, shown by fluorescence microscopy with an EC50 of 2300.0 nM. Despite its extensive bioactivities, 25-Hydroxycholesterol displays minimal cytotoxic and antitubercular activities against Vero cells and Mycobacterium tuberculosis H37Rv. It also has positive effects on reducing alpha-crystallin B R120G mutant protein aggregation in HeLa and HLE-B3 cells. Other notable activities include its impact on the SARS-CoV-2 3CL-Pro protease and an antiviral effect against SARS-CoV-2 induced cytotoxicity in Vero-6 cells. It also has a binding affinity to human integrin alphavbeta3 (Kd value of 10.9 nM) and degrades human HMGCR-dCat-ELuc in HEK293 cells with an EC50 of 630.0 nM. 25-Hydroxycholesterol displays a broad yet potent spectrum spanning several targets and pathways, making it a promising candidate for various therapeutic applications..

Note: Summary generated by AI. Data source: ChEMBL

生物活性

产品描述

25-Hydroxycholesterol is a steroid derivative that suppresses the cleavage of sterol regulatory element binding proteins (SREBPs). It inhibits HMG-CoA reductase and so it also plays a significant role in the in vivo regulation of cholesterol biosynthesis after an acute dietary cholesterol challenge. 25-hydroxycholesterol is a potent (EC50≈65 nM) and selective suppressor of IgA production by B cells.

别名

25-羟基胆甾醇, 25-羟基胆固醇

化学信息

分子量	402.65
分子式	C₂₇H₄₆O₂
CAS No.	2140-46-7
Smiles	C[C@H](CCCC(C)(C)O)[C@H]1CC[C@H]2[C@@H]3CC=C4C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C
密度	1.03g/cm3

储存&溶解度

存储

store at low temperature | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

溶解度信息

Ethanol: 20 mg/mL (49.67 mM), Sonication is recommended.

Chloroform: 50 mg/mL (124.18 mM), Sonication is recommended.

溶液配制表

Ethanol/Chloroform

	1mg	5mg	10mg	50mg
1 mM	2.4835 mL	12.4177 mL	24.8355 mL	124.1773 mL
5 mM	0.4967 mL	2.4835 mL	4.9671 mL	24.8355 mL
10 mM	0.2484 mL	1.2418 mL	2.4835 mL	12.4177 mL
20 mM	0.1242 mL	0.6209 mL	1.2418 mL	6.2089 mL

Chloroform

	1mg	5mg	10mg	50mg
50 mM	0.0497 mL	0.2484 mL	0.4967 mL	2.4835 mL
100 mM	0.0248 mL	0.1242 mL	0.2484 mL	1.2418 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：

比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10只，您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH₂O，那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 100 μL DMSO （母液浓度为 20 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法： 取 100 μL DMSO 母液，添加 400 μL PEG300 混匀澄清，再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH₂O 混匀澄清

以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。

方案所需的各类助溶剂如： DMSO， PEG300， PEG400， Tween 80， SBE-β-CD，玉米油等，均可在TargetMol网站点击购买。

剂量转换

对于不同动物的给药剂量换算，您也可以参考更多

关键词

Inhibitor inhibit HMGCoAReductase HMG-CoA Reductase HMGCoA Reductase EndogenousMetabolite Endogenous Metabolite 25-Hydroxycholesterol 25Hydroxycholesterol 25 Hydroxycholesterol

25-Hydroxycholesterol

25-Hydroxycholesterol