Mdivi-1 (Mitochondrial division inhibitor 1) is a mitochondrial division inhibitor that inhibits DRP1 and Dynamin I (IC50=1-10 μM). Mdivi-1 inhibits mitochondrial autophagy.

POP92:15 µM, MDA-MB-231 cells:55.93 ± 1.92 μM, Dnm1 GTPase:1 μM-10 μM

方法：人乳腺癌细胞 MDA-MB-231 用 Mdivi-1 (5-40 μM) 处理 8 h，使用 Immunostaining 方法分析有丝分裂纺锤体的异常。
结果：Mdivi-1 剂量依赖性地增加具有纺锤体异常的有丝分裂细胞的百分比。[1]
方法：人肺癌细胞 H460、A549 和人结直肠癌细胞 HCT116 用 Mdivi-1 (20-50 μM) 处理 24 h，使用 Flow Cytometry 方法检测细胞死亡情况。
结果：Mdivi-1 显著抑制三种肿瘤细胞的死亡。[2]

方法：为检测体内活性，将 Mdivi-1 (50 mg/kg) 腹腔注射给 C57BL/6 小鼠，随后通过急性眼压升高诱导短暂的视网膜缺血。
结果：Mdivi-1 治疗阻断了缺血视网膜中的凋亡细胞死亡，并显著提高了缺血后两周视网膜神经节细胞的存活率。[3]
方法：为研究对实验性自身免疫性脑脊髓炎 (EAE) 的影响，将 Mdivi-1 (25 mg/kg) 腹腔注射给诱导 EAE 的 C57BL/6 小鼠，每天一次，持续二十五天。
结果：Mdivi-1 通过减少中枢神经系统中的脱髓鞘和细胞浸润，有效地抑制了 EAE 的严重程度。Mdivi-1 通过调节 Th1/Th17 和调节性 T 细胞之间的平衡，在 EAE 中具有治疗潜力。[4]

All GTPase assay reactions are started in a 200 μL volume, of which 150 μL is placed into the well of a 96-well plate. Depletion of NADH, as monitored by reading the A340 of the reaction, is measured every 20 s for a total of 40 min using a SpectraMAX 250 96-well plate reader. Spectrophotometric data are transferred to Excel and the measured steady state depletion of NADH over time is converted to protein activity.

Mdivi-1 is dissolved in DMSO. YPGlycerol plates are topped with 10 mL YPGlycerol containing 1% low melt agar and 75 μM mdivi-1, and cells are spotted 12 hours later using a 48 well pinning device. After pinning cells, plates are incubated at 24°C or 37°C and imaged using an Eagle Eye II imaging system.