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Anti-TNF-alpha Antibody-FITC (7S35)
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产品编号 TMAY-01009F
Anti-TNF-alpha Antibody-FITC (7S35) 是一种 FITC-conjugated Mouse 抗体，靶向 TNF-alpha。Anti-TNF-alpha Antibody-FITC (7S35) 可用于 FCM。
Anti-TNF-alpha Antibody-FITC (7S35)
Anti-TNF-alpha Antibody-FITC (7S35)
还可以产品编号 TMAY-01009F
Anti-TNF-alpha Antibody-FITC (7S35) 是一种 FITC-conjugated Mouse 抗体，靶向 TNF-alpha。Anti-TNF-alpha Antibody-FITC (7S35) 可用于 FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 25 T | ¥ 695 | 5日内发货 | |
| 100 T | ¥ 1,490 | 5日内发货 |
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抗原信息
| 产品描述 | Anti-TNF-alpha Antibody-FITC (7S35) is a FITC-conjugated Mouse antibody targeting TNF-alpha. Anti-TNF-alpha Antibody-FITC (7S35) can be used in FCM. |
| Ig Type | Mouse IgG1 |
| 克隆号 | 7S35 |
| 交叉反应 | Human |
| 验证活性 | Flow cytometric analysis of anti-human TNFα on stimulated PBMC. Human peripheral blood mononuclear cells were stimulated for 6 hrs with Leukocyte Activation Cocktail, with BD GolgiPlug™ (BD Pharmingen™ Cat. No.550583). Cells were then fixed and permeabilized according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), followed by staining with Alexa Fluor® 647 Mouse Anti-Human CD8 (clone RPA-T8, BD Pharmingen™ Cat. No. 557708) and FITC Mouse anti-Human TNFα. The dot plots were derived from gated lymphocytes. |
| 应用 | |
| 推荐剂量 | 10 μl/Test, 0.1 mg/ml |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TNF-alpha (rh TNF-alpha; TMPY-00936; NP_000585.2; Val 77-Leu 233) and conjugated with FITC under optimum conditions, the unreacted FITC was removed. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | PBS solution containing 0.5% BSA and 0.1% sodium azide |
| 研究背景 | Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & AntibodyCancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| 偶联 | FITC |
| 其他可选修饰符 | Unconjugated |
| 可提供抗体种类 | 2 |
| 免疫原 | Recombinant Protein: Human TNF-alpha protein (TMPY-00936) |
| 抗原种属 | Human |
| 研究领域 | Cancer Drug Targets, Neuroinflammation |
存储&运输
| 储存方式 | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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