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Anti-TNF-alpha Antibody (7S35)
还可以产品编号 TMAY-01009
Anti-TNF-alpha Antibody (7S35) 是一种 Mouse 抗体，靶向 TNF-alpha。Anti-TNF-alpha Antibody (7S35) 可用于 ELISA,ELISA(Cap),FCM。
Anti-TNF-alpha Antibody (7S35)
Anti-TNF-alpha Antibody (7S35)
还可以产品编号 TMAY-01009
Anti-TNF-alpha Antibody (7S35) 是一种 Mouse 抗体，靶向 TNF-alpha。Anti-TNF-alpha Antibody (7S35) 可用于 ELISA,ELISA(Cap),FCM。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-TNF-alpha Antibody (7S35) is a Mouse antibody targeting TNF-alpha. Anti-TNF-alpha Antibody (7S35) can be used in ELISA,ELISA(Cap),FCM. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 7S35 |
| 交叉反应 | Human |
| 验证活性 | Flow cytometric analysis of Purified anti-Human TNFa on CHO transfected cells and stimulated lymphocytes. Left panel The transfected cells stained with Purified Mouse anti-TNFa (Bold line hisgram), To demonstrate specificity of staining, the binding of Anti-TNF-alpha Antibody was blocked by the preincubation of the purified antibody with molar excess of recombinant human TNFa (5 μg) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody. Right panel Human PBMC were stimulated for 4 hrs with Leukocyte Activation Cocktail (with BD GolgiPlug™, BD Pharmingen™ Cat. No. 550583), then cells were harvested and treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714). |
| 应用 | ELISA,ELISA(Cap),FCM |
| 推荐剂量 | ELISA: 1:1000-1:2000; FCM: 1:25-1:100; ELISA(Cap): 1:250-1:2000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Tumor necrosis factor-alpha (rhTNFα; TMPY-00936; Val 77-Leu 233; NP_000585.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & AntibodyCancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| 偶联 | Unconjugated |
| 其他可选修饰符 | FITC |
| 可提供抗体种类 | 2 |
| 免疫原 | Recombinant Human TNFα protein (TMPY-00936) |
| 抗原种属 | Human |
| 研究领域 | Cancer Drug Targets, Neuroinflammation |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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