| Powder: -20°C for 3 years | In solvent: -80°C for 2 years
Bleomycin Sulfate 是一种 DNA 损伤剂,抑制DNA 合成。它还是一种抗肿瘤的抗生素。
产品描述 | Bleomycin Sulfate, a glycopeptide antibiotic, is an anticancer agent for squamous cell carcinomas (SCC). In UT-SCC-19A cells, the IC50 of Bleomycin Sulfate is 4 nM. |
靶点活性 | Bleomycin Sulfate:4 nM |
体内活性 | In a Bleomycin-induced mouse model, BLM was found to promote the development of inflammation, with the increase of TGF-β1, Smad3, and STAT1 leading to severe pulmonary fibrosis. |
细胞实验 | ADIPO-P2 cells are grown in D-MEM high glucose medium supplemented with 20% fetal calf serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 37 °C and 5% CO2 atmosphere. Cells are cultured as monolayer in TC25 Corning flasks containing 1.5 × 105 cells/mL. For each experiment, two flasks are set up, one for the control and one for the treated culture. During the log phase of growth ADIPO-P2 cells are treated with a 30 minutes pulse of 2.5 μg/mL of Bleomycin sulfate. Control cultures are set up in parallel but not exposed to Bleomycin sulfate. Time of exposure and concentration of Bleomycin sulfate are chosen according to previous studies carried out in our laboratory with mammalian cells exposed to Bleomycin sulfate. At the end of the pulse treatment with Bleomycin sulfate, the cells are washed twice with Hank's balanced salt solution and kept in culture with fresh culture medium until harvesting. Cells are continuously maintained in culture during 5 passages or subcultures after treatment. Subcultivation is carried out whenever the cultures became confluent (approximately 4 × 105 cells/mL of culture medium). To estimate cell growth, at the time of subcultivation cells are collected by trypsinization, an aliquot of about 200 μL stained with 0.4% trypan blue, and the number of viable cells is determined. Cells are then suspended in fresh culture medium and dispensed into new culture flasks containing 1 × 105 cells/mL to continue growing. The rest of the cells is discarded or dispensed in another flask for cytogenetic analysis, which is performed at 18 hours and 10 days after the end of treatments. To analyze chromosomal aberrations, colchicine (0.1 μg/mL) is added to cell cultures during the last 3 hours of culture. Chromosome preparations are made following standard procedures. After harvesting, cells are hypotonically shocked, fixed in methanol:acetic acid (3:1), spread onto glass slides and processed for PNA-FISH.Two independent experiments are carried out. (Only for Reference) |
别名 | Blenoxane, 硫酸博来霉素, 硫酸博莱霉素, NSC125066 |
化合物与蛋白结合的复合物 |
Crystal structure of bleomycin-binding protein from bleomycin-producing Streptomyces verticillus complexed with metal-free bleomycin |
分子量 | 1512.62 |
分子式 | C55H85N17O25S4 |
CAS No. | 9041-93-4 |
| Powder: -20°C for 3 years | In solvent: -80°C for 2 years
Ethanol: <1 mg/mL
H2O: 92 mg/mL (60.8 mM)
DMSO: 93 mg/mL (61.5 mM)
( < 1 mg/mL refers to the product slightly soluble or insoluble )
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
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您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Bleomycin Sulfate 9041-93-4 Cell Cycle/Checkpoint DNA Damage/DNA Repair Microbiology/Virology Antibiotic DNA/RNA Synthesis inhibit Blenoxane 硫酸博来霉素 硫酸博莱霉素 Bleomycin NSC125066 Inhibitor inhibitor