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Bleomycin Sulfate

产品编号 T6116 CAS 9041-93-4

别名: Blenoxane, 硫酸博来霉素, 硫酸博莱霉素, NSC125066

Bleomycin Sulfate 是一种 DNA 损伤剂，抑制DNA 合成。它还是一种抗肿瘤的抗生素。

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Bleomycin Sulfate, CAS 9041-93-4

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其他形式的 Bleomycin Sulfate:

Bleomycin hydrochloride
现货

选择批次

纯度: 100%

纯度: 99.73%

纯度: 99.67%

纯度: 98%

更多批次查询请联系客服

天然产物信息

生物活性

化学信息

存储 & 溶解度

参考文献

相关产品

植物来源	天然产物 > 其他（植物来源） > 其他

产品描述	Bleomycin Sulfate, a glycopeptide antibiotic, is an anticancer agent for squamous cell carcinomas (SCC). In UT-SCC-19A cells, the IC50 of Bleomycin Sulfate is 4 nM.
靶点活性	Bleomycin Sulfate:4 nM
体内活性	In a Bleomycin-induced mouse model, BLM was found to promote the development of inflammation, with the increase of TGF-β1, Smad3, and STAT1 leading to severe pulmonary fibrosis.
细胞实验	ADIPO-P2 cells are grown in D-MEM high glucose medium supplemented with 20% fetal calf serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 37 °C and 5% CO2 atmosphere. Cells are cultured as monolayer in TC25 Corning flasks containing 1.5 × 105 cells/mL. For each experiment, two flasks are set up, one for the control and one for the treated culture. During the log phase of growth ADIPO-P2 cells are treated with a 30 minutes pulse of 2.5 μg/mL of Bleomycin sulfate. Control cultures are set up in parallel but not exposed to Bleomycin sulfate. Time of exposure and concentration of Bleomycin sulfate are chosen according to previous studies carried out in our laboratory with mammalian cells exposed to Bleomycin sulfate. At the end of the pulse treatment with Bleomycin sulfate, the cells are washed twice with Hank's balanced salt solution and kept in culture with fresh culture medium until harvesting. Cells are continuously maintained in culture during 5 passages or subcultures after treatment. Subcultivation is carried out whenever the cultures became confluent (approximately 4 × 105 cells/mL of culture medium). To estimate cell growth, at the time of subcultivation cells are collected by trypsinization, an aliquot of about 200 μL stained with 0.4% trypan blue, and the number of viable cells is determined. Cells are then suspended in fresh culture medium and dispensed into new culture flasks containing 1 × 105 cells/mL to continue growing. The rest of the cells is discarded or dispensed in another flask for cytogenetic analysis, which is performed at 18 hours and 10 days after the end of treatments. To analyze chromosomal aberrations, colchicine (0.1 μg/mL) is added to cell cultures during the last 3 hours of culture. Chromosome preparations are made following standard procedures. After harvesting, cells are hypotonically shocked, fixed in methanol:acetic acid (3:1), spread onto glass slides and processed for PNA-FISH.Two independent experiments are carried out. (Only for Reference)

别名	Blenoxane, 硫酸博来霉素, 硫酸博莱霉素, NSC125066
化合物与蛋白结合的复合物	Crystal structure of bleomycin-binding protein from bleomycin-producing Streptomyces verticillus complexed with metal-free bleomycin
分子量	1512.62
分子式	C55H85N17O25S4
CAS No.	9041-93-4

存储

　|　Powder: -20°C for 3 years | In solvent: -80°C for 2 years

溶解度

Ethanol: <1 mg/mL

H2O: 92 mg/mL (60.8 mM)

DMSO: 93 mg/mL (61.5 mM)

( < 1 mg/mL refers to the product slightly soluble or insoluble )

参考文献

1. Jääskelä-Saari HA, et al. Acta Otolaryngol Suppl. 1997;529:241-4. 2. Denholm EM, et al. Am J Pathol. 1989, 134(2), 355-363. 3. Cort A, et al. Mol Med Report. 2012, 5(6), 1481-1486. 4. Paviolo NS, et al. Mutat Res. 2012, 734(1-2), 5-11. 5. Banerjee ER, et al. Stem Cell Res Ther. 2012, 3(3), 21. 6. Hovhannisyan G, et al. Comparative analysis of individual chromosome involvement in micronuclei induced by bleomycin in human leukocytes. Mol Cytogenet. 2016 Jun 21;9:49. 7. Corboz MR, et al. Therapeutic administration of inhaled INS1009, a treprostinil prodrug formulation, inhibits bleomycin-induced pulmonary fibrosis in rats. Pulm Pharmacol Ther. 2018 Apr;49:95-103. 8. Liang J, Niu Z, Yu X, et al. Counteracting Genome Instability by p53-dependent Mintosis[J]. bioRxiv. 2020. 9. Zongwang Zhang1,2 , Yanwei Wu2 , Bing Wu2,3, etal, Lili Niu1* and Wei Tang2,3. DZ2002 ameliorates fibrosis, inflammation,and vasculopathy in experimental systemic sclerosis models. Arthritis Research & Therapy. (2019) 21:290 10. Liang J, Niu Z, Zhang B, et al. Liang J, Niu Z, Zhang B, et al. p53-dependent elimination of aneuploid mitotic offspring by entosis[J]. Cell Death & Differentiation. 2020: 1-15.

文献引用

1. Yi X M, Li M, Chen Y D, et al. Reciprocal regulation of IL-33 receptor–mediated inflammatory response and pulmonary fibrosis by TRAF6 and USP38. Proceedings of the National Academy of Sciences. 2022, 119(10): e2116279119 2. Liang J, Niu Z, Zhang B, et al. p53-dependent elimination of aneuploid mitotic offspring by entosis. Cell Death & Differentiation. 2020: 1-15 3. Liang J, Niu Z, Zhang B, et al Liang J, Niu Z, Zhang B, et al. p53-dependent elimination of aneuploid mitotic offspring by entosis. Cell Death & Differentiation. 2020: 1-15. 4. Wang T, Shi S, Shi Y, et al.Chemical-induced phase transition and global conformational reorganization of chromatin.Nature Communications.2023, 14(1): 5556.

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

剂量

mg/kg

每只动物体重

给药体积

μL

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

计算重置

计算结果如下:

工作液浓度: mg/ml;

母液配置方法: mg 药物溶于 μL DMSO (母液浓度为 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 μL DMSO 主液，加入 μL PEG300，混匀澄清，再加 μL Tween 80，混匀澄清，再加 μL ddH₂O, 混匀澄清。

备注:

要按顺序从左向右依次添加助溶剂。可配合物理方法，如涡流、超声波或热水浴使之帮助溶解。

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

质量

浓度

容积

分子量

浓度 (起始)

容积 (起始)

浓度 (终)

容积 (终)

溶液体积

质量

配液浓度

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技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到，包括如何准备库存溶液，如何存储产品，以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Bleomycin Sulfate 9041-93-4 Cell Cycle/Checkpoint DNA Damage/DNA Repair Microbiology/Virology Antibiotic DNA/RNA Synthesis inhibit Blenoxane 硫酸博来霉素硫酸博莱霉素 Bleomycin NSC125066 Inhibitor inhibitor

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Bleomycin Sulfate

存储

溶解度

参考文献

文献引用

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剂量换算

体内实验配液计算器

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输入分子式，点击计算，可计算出产品的分子量。

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