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Bleomycin Sulfate

Bleomycin Sulfate

产品编号 T6116   CAS 9041-93-4
别名: Blenoxane, 硫酸博来霉素, 硫酸博莱霉素, NSC125066

Bleomycin Sulfate 是一种 DNA 损伤剂,抑制DNA 合成。它还是一种抗肿瘤的抗生素。

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Bleomycin Sulfate, CAS 9041-93-4
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其他形式的 Bleomycin Sulfate:
产品目录号及名称: Bleomycin Sulfate (T6116)
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纯度: 100%
纯度: 99.73%
纯度: 99.67%
纯度: 98%
纯度: 98%
纯度: 98%
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天然产物信息
生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 Bleomycin Sulfate, a glycopeptide antibiotic, is an anticancer agent for squamous cell carcinomas (SCC). In UT-SCC-19A cells, the IC50 of Bleomycin Sulfate is 4 nM.
靶点活性 Bleomycin Sulfate:4 nM
体内活性 In a Bleomycin-induced mouse model, BLM was found to promote the development of inflammation, with the increase of TGF-β1, Smad3, and STAT1 leading to severe pulmonary fibrosis.
细胞实验 ADIPO-P2 cells are grown in D-MEM high glucose medium supplemented with 20% fetal calf serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 37 °C and 5% CO2 atmosphere. Cells are cultured as monolayer in TC25 Corning flasks containing 1.5 × 105 cells/mL. For each experiment, two flasks are set up, one for the control and one for the treated culture. During the log phase of growth ADIPO-P2 cells are treated with a 30 minutes pulse of 2.5 μg/mL of Bleomycin sulfate. Control cultures are set up in parallel but not exposed to Bleomycin sulfate. Time of exposure and concentration of Bleomycin sulfate are chosen according to previous studies carried out in our laboratory with mammalian cells exposed to Bleomycin sulfate. At the end of the pulse treatment with Bleomycin sulfate, the cells are washed twice with Hank's balanced salt solution and kept in culture with fresh culture medium until harvesting. Cells are continuously maintained in culture during 5 passages or subcultures after treatment. Subcultivation is carried out whenever the cultures became confluent (approximately 4 × 105 cells/mL of culture medium). To estimate cell growth, at the time of subcultivation cells are collected by trypsinization, an aliquot of about 200 μL stained with 0.4% trypan blue, and the number of viable cells is determined. Cells are then suspended in fresh culture medium and dispensed into new culture flasks containing 1 × 105 cells/mL to continue growing. The rest of the cells is discarded or dispensed in another flask for cytogenetic analysis, which is performed at 18 hours and 10 days after the end of treatments. To analyze chromosomal aberrations, colchicine (0.1 μg/mL) is added to cell cultures during the last 3 hours of culture. Chromosome preparations are made following standard procedures. After harvesting, cells are hypotonically shocked, fixed in methanol:acetic acid (3:1), spread onto glass slides and processed for PNA-FISH.Two independent experiments are carried out. (Only for Reference)
别名 Blenoxane, 硫酸博来霉素, 硫酸博莱霉素, NSC125066
化合物与蛋白结合的复合物

T6116_2

Crystal structure of bleomycin-binding protein from bleomycin-producing Streptomyces verticillus complexed with metal-free bleomycin

分子量 1512.62
分子式 C55H85N17O25S4
CAS No. 9041-93-4

存储

 | Powder: -20°C for 3 years | In solvent: -80°C for 2 years

溶解度

Ethanol: <1 mg/mL

H2O: 92 mg/mL (60.8 mM)

DMSO: 93 mg/mL (61.5 mM)

( < 1 mg/mL refers to the product slightly soluble or insoluble )

参考文献

1. Jääskelä-Saari HA, et al. Acta Otolaryngol Suppl. 1997;529:241-4. 2. Denholm EM, et al. Am J Pathol. 1989, 134(2), 355-363. 3. Cort A, et al. Mol Med Report. 2012, 5(6), 1481-1486. 4. Paviolo NS, et al. Mutat Res. 2012, 734(1-2), 5-11. 5. Banerjee ER, et al. Stem Cell Res Ther. 2012, 3(3), 21. 6. Hovhannisyan G, et al. Comparative analysis of individual chromosome involvement in micronuclei induced by bleomycin in human leukocytes. Mol Cytogenet. 2016 Jun 21;9:49. 7. Corboz MR, et al. Therapeutic administration of inhaled INS1009, a treprostinil prodrug formulation, inhibits bleomycin-induced pulmonary fibrosis in rats. Pulm Pharmacol Ther. 2018 Apr;49:95-103. 8. Liang J, Niu Z, Yu X, et al. Counteracting Genome Instability by p53-dependent Mintosis[J]. bioRxiv. 2020. 9. Zongwang Zhang1,2 , Yanwei Wu2 , Bing Wu2,3, etal, Lili Niu1* and Wei Tang2,3. DZ2002 ameliorates fibrosis, inflammation,and vasculopathy in experimental systemic sclerosis models. Arthritis Research & Therapy. (2019) 21:290 10. Liang J, Niu Z, Zhang B, et al. Liang J, Niu Z, Zhang B, et al. p53-dependent elimination of aneuploid mitotic offspring by entosis[J]. Cell Death & Differentiation. 2020: 1-15.

文献引用

1. Yi X M, Li M, Chen Y D, et al. Reciprocal regulation of IL-33 receptor–mediated inflammatory response and pulmonary fibrosis by TRAF6 and USP38. Proceedings of the National Academy of Sciences. 2022, 119(10): e2116279119 2. Liang J, Niu Z, Zhang B, et al. p53-dependent elimination of aneuploid mitotic offspring by entosis. Cell Death & Differentiation. 2020: 1-15 3. Liang J, Niu Z, Zhang B, et al Liang J, Niu Z, Zhang B, et al. p53-dependent elimination of aneuploid mitotic offspring by entosis. Cell Death & Differentiation. 2020: 1-15. 4. Wang T, Shi S, Shi Y, et al.Chemical-induced phase transition and global conformational reorganization of chromatin.Nature Communications.2023, 14(1): 5556.
6-Diazo-5-oxo-L-nor-Leucine Trovafloxacin Nanchangmycin Salinomycin sodium salt Eravacycline HCl Halazone T.cruzi-IN-1 Amorolfine HCL

相关化合物库

该产品包含在如下化合物库中:
抗癌化合物库 上市药物库 多肽分子库 抗衰老化合物库 抗感染化合物库 药物功能重定位化合物库 FDA 上市药物库 NO PAINS 化合物库 抗生素库

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体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

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Keywords

Bleomycin Sulfate 9041-93-4 Cell Cycle/Checkpoint DNA Damage/DNA Repair Microbiology/Virology Antibiotic DNA/RNA Synthesis inhibit Blenoxane 硫酸博来霉素 硫酸博莱霉素 Bleomycin NSC125066 Inhibitor inhibitor

 

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