Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Anisomycin (NSC-76712) 是一种从各种链霉菌属中分离出来的抗生素。 它通过抑制肽基转移酶或 80S 核糖体系统来干扰蛋白质和 DNA 合成。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 mg | ¥ 415 | 现货 | ||
25 mg | ¥ 582 | 现货 | ||
50 mg | ¥ 828 | 现货 | ||
100 mg | ¥ 1,467 | 现货 | ||
200 mg | ¥ 2,050 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 410 | 现货 |
产品描述 | Anisomycin (NSC-76712) is an antibiotic isolated from various Streptomyces species. It interferes with protein and DNA synthesis by inhibiting peptidyl transferase or the 80S ribosome system. |
体外活性 | Anisomycin (3 μM) decreases protein synthesis in MDA16 and MDA-MB-468 cells, and reduces colony formation by MDA-MB-468 cells. Anisomycin causes an increase in the number of apoptotic cells in MDA-MB-468 cultures, but not in MDA16 cultures. Anisomycin actives JNK phosphorylation in MDA-MB-468 cells.[2] In U251 and U87 cells, anisomycin?(0.01-8 μM) inhibits the cell growth in time- and concentration-dependent manners with the IC50 (48 h) values of 0.233 and 0.192 μmol/L, respectively. Anisomycin?(4 μM) causes 21.5% and 25.3% of apoptosis proportion in U251 and U87 cells, respectively, and activates p38 MAPK and?JNK, while inactivated ERK1/2. Anisomycin?(4 μM) reduces the level of PP2A/C subunit in a time-dependent manner in U251 and U87 cells.[3] Anisomycin inhibits EAC cell proliferation in concentration-dependent manner.[4] |
体内活性 | Peritumoral administration of anisomycin (5 mg/kg) significantly suppresses Ehrlich ascites carcinoma (EAC) growth resulting in the survival of approximately 60% of the mice 90 days after EAC inoculation.[4] |
激酶实验 | JNK phosphorylation: 500,000 cells/well are seeded in 6-well plates and incubated overnight. Cells are then incubated for 1 h with test compounds or DMSO as vehicle control (?nal concentration 1% v/v). Puromycin is added (?nal concentration of 18 μM) and cells incubated for a further 10 min to label nascent polypeptide chains. Background labelling is determined by incubating cells without puromycin. Cells are then washed in HBSS, harvested by scraping and centrifuged (300 g, 5 min). Cells are resuspended in 0.5 mL 50 mM DTT containing phosphatase inhibitors and incubated at 95℃ for 10 min. Samples are then snap frozen in liquid nitrogen and stored at -20℃ until blotted. Samples (20–30 μg protein/sample) are blotted onto a PVDF membrane. The membrane is blocked and incubated with anti-phospho-Thr183/Tyr185-JNK antibody overnight at 4℃. Secondary antibodies are used to label the primary antibody and detected using an infrared scanner. The intensity of the ?uorescence signal for anti-phospho-JNK antibody is background corrected and normalized for loading. |
细胞实验 | For the assay, EAC cells are plated in 96-well plates at a density of 10,000 cells/well/200 μL of medium. The cells are treated with the different concentrations of anisomycin for 48 h. Adriamycin (500 ng/mL) is used as a positive control. 0.5 mg/mL of MTT is added to each well. 4 h later, the formazan product of MTT reduction is dissolved in DMSO, and absorbance is measured at 570 nm using a Model 680 microplate reader.(Only for Reference) |
别名 | 茴香霉素, Flagecidin, NSC 76712, Wuningmeisu C |
化合物与蛋白结合的复合物 |
Co-crystal Structure of Anisomycin Bound to the 50S Ribosomal Subunit |
分子量 | 265.3 |
分子式 | C14H19NO4 |
CAS No. | 22862-76-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 13.3 mg/mL (50 mM)
DMSO: 26.5 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 3.7693 mL | 18.8466 mL | 37.6932 mL | 94.2329 mL |
5 mM | 0.7539 mL | 3.7693 mL | 7.5386 mL | 18.8466 mL | |
10 mM | 0.3769 mL | 1.8847 mL | 3.7693 mL | 9.4233 mL | |
20 mM | 0.1885 mL | 0.9423 mL | 1.8847 mL | 4.7116 mL | |
50 mM | 0.0754 mL | 0.3769 mL | 0.7539 mL | 1.8847 mL | |
DMSO | 100 mM | 0.0377 mL | 0.1885 mL | 0.3769 mL | 0.9423 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Anisomycin 22862-76-6 Apoptosis Cell Cycle/Checkpoint DNA Damage/DNA Repair MAPK Microbiology/Virology Antibiotic JNK Antibacterial DNA/RNA Synthesis Bacterial Inhibitor 茴香霉素 NSC-76712 Flagecidin NSC 76712 inhibit Wuningmeisu C NSC76712 inhibitor