apf

APF is an aromatic amino-fluorescein derivative and fluorescent probe for highly reactive radicals. It has low intrinsic fluorescence, however, upon oxidation by hydroxyl radical, hypochlorite ion, and certain peroxidase intermediates, it is converted to the highly fluorescent molecule fluorescein, facilitating the detection of highly reactive biological radicals. APF is not oxidized by nitric oxide (NO), hydrogen peroxide (H2O2), or other oxidants. Fluorescein displays excitation/emission maxima of 490/515 nm, respectively.

Suc-AAPF-pNA (琥珀酰-AAPF-对硝基苯胺) 是一种底物显色剂，裂解后释放出黄色的4-硝基苯胺，是许多蛋白酶和前列腺特异性抗原（PA）的底物，可以用于鉴定酵母突变体中新的蛋白水解活性。