Anti-WFDC2 Antibody (7A490) is an antibody targeting WFDC2. Anti-WFDC2 Antibody (7A490) can be used in ELISA, WB, FC.

Rabbit IgG

7A490

Human

1. Western Blot
-Positive WB detected in: HT29 whole cell lysate, Hela whole cell lysate, HT29 whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, HEPG2 whole cell lysate
-All lanes: HE4 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 25 kDa
-Observed band size: 25 kDa
2. Overlay Peak curve showing MCF-7 cells stained with TMAH-01250 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, FC

WB:1:500-1:2000; FC:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human WFDC2

Human

10406

Tags & Cell Markers