Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Sphingosine-1-phosphate (S1P) 是 S1P1-5 受体 (S1P1-5 receptors) 的激动剂和 GPR3、GPR6、GPR12 的配体。Sphingosine-1-phosphate 是细胞内的第二信使,动员 Ca2+ 作为 G 蛋白偶联受体的细胞外配体。Sphingosine-1-phosphate 是由鞘磷脂 (HY-113498) 或其他膜磷脂生成的重要脂质介质。
产品描述 | Sphingosine-1-phosphate (S1P) is an agonist for S1P1-5 receptors and a ligand for GPR3, GPR6, GPR12.Sphingosine-1-phosphate is an intracellular second messenger that mobilises Ca2+ as an extracellular ligand for G-protein coupled receptors. Sphingosine-1-phosphate is an important lipid mediator generated from sphingosine (HY-113498) or other membrane phospholipids. |
体外活性 |
Sphingosine-1-phosphate (S1P) (1 μM) induces significant Ca2+ release in HEK293 cells under serum starvation conditions (1% FCS).[1]
In functional Ca2+ assays, Suramin alone had no effect on intracellular Ca2+ release via gpr3, gpr6, or gpr12. In contrast, S1P (1 μM) induces Ca2+ release from gpr3, gpr6, and gpr12 in transfected HEK293 cells in the presence of varying concentrations of suramin.[2] In a functional Ca2+ assay, S1P (3-3000 nM) exhibits nanomolar EC50 values in the presence of Suramin (300 μM) for gpr3 (EC50=29 nM), gpr6 (EC50=15 nM) and gpr12 (EC50=24 nM ), rat gpr3 (EC50=68 nM), respectively act on HEK293 cells.[2] S1P increases the expression of S1P1 and Gqi5, and when used at a concentration of 200 nM, it allows Gi proteins to stimulate phospholipase C, as well as TAg-Jurkat cells expressing S1P2 and S1P3 receptors (EC 50s=8 and 11 nM).[3] |
别名 | S1P |
分子量 | 379.47 |
分子式 | C18H38NO5P |
CAS No. | 26993-30-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NaOH: 37.95 mg/mL (100 mM), when pH is adjusted to 12 with 1 M NaOH. Sonication is recommended
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
NaOH | 1 mM | 2.6353 mL | 13.1763 mL | 26.3525 mL | 65.8814 mL |
5 mM | 0.5271 mL | 2.6353 mL | 5.2705 mL | 13.1763 mL | |
10 mM | 0.2635 mL | 1.3176 mL | 2.6353 mL | 6.5881 mL | |
20 mM | 0.1318 mL | 0.6588 mL | 1.3176 mL | 3.2941 mL | |
50 mM | 0.0527 mL | 0.2635 mL | 0.5271 mL | 1.3176 mL | |
100 mM | 0.0264 mL | 0.1318 mL | 0.2635 mL | 0.6588 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Sphingosine-1-phosphate 26993-30-6 GPCR/G Protein Metabolism S1P Receptor Endogenous Metabolite LPL Receptor S1P Inhibitor inhibitor inhibit