规格

价格

库存

数量

1 mg

¥ 578

现货

2 mg

¥ 853

现货

5 mg

¥ 1,450

现货

10 mg

¥ 1,910

现货

25 mg

¥ 3,270

现货

50 mg

¥ 4,680

现货

100 mg

¥ 6,530

现货

1 mL x 10 mM (in DMSO)

¥ 1,830

现货

生物活性

产品描述	Ophiopogonin D is a natural product,and is a CYP2J3 inducer that significantly inhibits Ang II induced NF-κB nuclear translocation. Ophiopogonin-D significantly inhibited the in vitro and in vivo growth of prostate cells via RIPK1. Ophiopogonin-D may be developed as a potential anti-prostate cancer agent.
体外活性	Ophiopogonin D(OPD) 对PC3细胞展现了强大的抗肿瘤活性。通过RIPK1相关途径诱导细胞凋亡，提高了RIPK1和Bim的蛋白表达水平，降低了cleaved-RIPK1、caspase 8、cleaved-caspase 8、Bid、caspase 10、及cleaved-caspase 10的水平。OPD还增加了Bim的mRNA表达。当细胞预先用necrostatin-1处理时，Bim的蛋白表达水平降低。[1] OPHIOPOGONIN D（OP-D）显著抑制了由Ang II引起的NF-κB核内转移、IκBα下调以及促炎细胞因子（TNF-α、IL-6和VCAM-1）的激活，这一过程是通过增加CYP2J2/EETs和PPARα在HUVECs中的表达来实现的。此外，外源性11,12-EET的处理减轻了Ang II引起的内皮细胞炎症反应，这表现为NF-κB核内转移的抑制，IκBα表达的增加以及炎症因子水平的降低。另外，Ang II诱导的NF-κB核内转移的激活也被非诺贝特显著抑制。在化合物处理前与6-(2-丙炔基氧基苯基)己酸（PPOH）和PPARα抑制剂GW6471共孵育，消除了OP-D对内皮的保护效果。这表明OP-D通过激活CYP2J和增加EETs以及PPARα的参与，发挥对内皮的保护作用。
体内活性	Ophiopogonin D(OPD)′ 以5mg/kg剂量治疗，自治疗第6天起显著抑制肿瘤生长（p = 0.034）。研究结束时（第24天），切除、拍照并称重肿瘤组织。以5mg/kg OPD′治疗结果在第24天与对照治疗相比，肿瘤生长抑制显著（p = 0.000），约79.8%。5.0 mg/kg剂量的肿瘤生长抑制作用明显强于2.5 mg/kg剂量（p = 0.000）。所有组别均未发现显著的体重减轻[1]。
细胞实验	The CCK-8 assay was used to assess the viability of prostate cancer cells.?The cell morphology was examined by an ultrastructural analysis via transmission electron microscopy.?Cells in apoptosis (early and late stages) were detected using an Annexin V-FITC/propidium iodide kit with a FACSCaliber flow cytometer.?JC-1, a cationic lipophilic probe, was employed to measure the mitochondrial membrane potential (MMP) of PC3 cells.?Changes in the protein expression of RIPK1, C-RIPK1, caspase 8, cleaved-caspase 8, Bim, Bid, caspase 10, and cleaved-caspase 10 were evaluated by Western blotting.?The mRNA expression of Bim was examined by quantitative real-time reverse transcription polymerase chain reaction.?Z-VAD-FMK (a caspase inhibitor) and necrostatin-1 (a specific inhibitor of RIPK1) were utilized to determine whether the cell death was mediated by RIPK1 or caspases[1].
动物实验	One week after tumor cell inoculation, mice bearing palpable tumors were randomly divided into control and treatment groups (8 mice/group).?OPD′ was dissolved in the vehicle, PEG400:Saline:Ethanol (400:300:200, v/v/v), and administered (via i.p. injection) at doses of 2.5 or 5.0 mg/kg bodyweight 5 days a week for 24 days.?The control group received vehicle only.?The mice were sacrificed by cervical dislocation on Day 24, and the tumor tissues were removed and weighed[1].
别名	麦冬皂苷D, 41753-55-3

化学信息

分子量	855.02
分子式	C₄₄H₇₀O₁₆
CAS No.	945619-74-9
Smiles	C[C@@]12[C@@]3([C@](C[C@]1([C@]4([C@](CC2)([C@@]5(C)[C@H](O[C@H]6[C@H](O[C@H]7[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O7)[C@@H](O[C@H]8[C@H](O)[C@@H](O)[C@H](O)CO8)[C@@H](O)[C@@H](C)O6)C[C@H](O)CC5=CC4)[H])[H])[H])(O[C@@]9([C@H]3C)CC[C@@H](C)CO9)[H])[H]
密度	1.4

储存&溶解度

存储

Keep away from direct sunlight,Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

实际储存温度请以COA为准

溶解度信息

DMSO: 140 mg/mL (163.74 mM), Sonication is recommended.

体内实验配方

10% DMSO+90% Saline: 10 mg/mL (11.7 mM), Suspension.

10% DMSO+90% Corn Oil: 2 mg/mL (2.34 mM)

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.1696 mL	5.8478 mL	11.6956 mL	58.4782 mL
5 mM	0.2339 mL	1.1696 mL	2.3391 mL	11.6956 mL
10 mM	0.1170 mL	0.5848 mL	1.1696 mL	5.8478 mL
20 mM	0.0585 mL	0.2924 mL	0.5848 mL	2.9239 mL
50 mM	0.0234 mL	0.1170 mL	0.2339 mL	1.1696 mL
100 mM	0.0117 mL	0.0585 mL	0.1170 mL	0.5848 mL

该溶液配制表仅适用于固体产品。对于液体产品，请根据标明的浓度或密度计算稀释方案。

Ophiopogonin-D

Ophiopogonin-D

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词

Ophiopogonin-D

Ophiopogonin-D

Ophiopogonin-D 相关产品

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词