Anti-ROS1 Polyclonal Antibody is a Rabbit antibody targeting ROS1. Anti-ROS1 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Rat

1. Sample:
Lane 1: Cerebrum (Rat) Lysate at 40 μg
Lane 2: Cerebellum (Rat) Lysate at 40 μg
Lane 3: Pituitary (Rat) Lysate at 40 μg
Lane 4: Siha (Human) Cell Lysate at 30 μg
Lane 5: U251 (Human) Cell Lysate at 30 μg
Primary: Anti-ROS1 (TMAB-01635) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 258 kDa
Observed band size: >250/110 kDa
2. Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-ROS1 antibody (TMAB-01635)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human ROS1

Human

ROS1

Proto-oncogene tyrosine-protein kinase ROS

Growth factors,Proto-oncogenes,Receptor Tyrosine Kinases

Orphan receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2.