NB-598 is an effective and competitive inhibitor of squalene epoxidase. It suppresses triglyceride biosynthesis through the farnesol pathway.

Caco-2 cells are grown in a 58 cm2 plastic dish with medium A for 13 days. The cells are washed with medium B, and then cultured with medium B including cholesterol-micelle and each compound. The compound is dissolved in Me2SO, and the final concentration of Me2SO is 0.1%(v/v). After 18 hr of incubation, the cells are washed extensively with phosphate-buffered saline (PBS) to remove the compound. Microsomes are prepared as described above. The reaction mixture (0.2 mL) consisted of 0.1 mg microsomes, 0.25% BSA and 40 PM [14C]oleoyl CoA in buffer A. To avoid the effects of endogenous cholesterol, liposome (2 mol of cholesterol: 1 mol of phosphatidylcholine) [15] is added to the reaction mixture. The microsomes are preincubated for 1 hr with or without exogenous cholesterol, and ACAT activity is determined as described above [1].

DMSO: 140 mg/mL (311.34 mM), Sonication is recommended.

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 10 mg/mL (22.24 mM), Solution.

10% DMSO+90% Corn Oil: 1 mg/mL (2.22 mM), Sonication is recommended.

10% DMSO+90% Saline: < 10 mg/mL (22.24 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.