store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ionomycin 是一种钙离子载体和一种结合钙离子(Ca2+)的抗生素。它是由联合链霉菌(Streptomyces conglobatus)产生的。它在研究中用于提高细胞内钙水平(Ca2+),并作为了解 Ca2+跨生物膜运输的研究工具。Ionomycin 可促进细胞凋亡,诱导蛋白激酶 C (PKC)活化。
产品描述 | Ionomycin is an calcium ionophore and an antibiotic that binds calcium ions (Ca2+). It is produced by the bacterium Streptomyces conglobatus.It is used in research to raise the intracellular calcium level (Ca2+) and as a research tool to understand Ca2+ transport across biological membranes. Ionomycin promotes apoptosis and induces the activation of protein kinase C (PKC). |
体外活性 | Ionomycin (2 μM) induced cell death in LCLC 103H cells, derived from a human large cell lung carcinoma, by inducing calpain activation promotes decrease of Bcl-2 proteins thereby triggering the intrinsic apoptotic pathway [2].Ionomycin also phosphorylate p38 MAPK by Ca2+ influx through SOCE, leading to suppression of TNF-α-induced NF-κB phosphorylation[3]. |
分子量 | 709.01 |
分子式 | C41H72O9 |
CAS No. | 56092-81-0 |
store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 90.0 mg/mL (126.9 mM), Sonication is recommended.
DMSO: 90.0 mg/mL (126.9 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 1.4104 mL | 7.0521 mL | 14.1042 mL | 35.2604 mL |
5 mM | 0.2821 mL | 1.4104 mL | 2.8208 mL | 7.0521 mL | |
10 mM | 0.141 mL | 0.7052 mL | 1.4104 mL | 3.526 mL | |
20 mM | 0.0705 mL | 0.3526 mL | 0.7052 mL | 1.763 mL | |
50 mM | 0.0282 mL | 0.141 mL | 0.2821 mL | 0.7052 mL | |
100 mM | 0.0141 mL | 0.0705 mL | 0.141 mL | 0.3526 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Ionomycin 56092-81-0 Membrane transporter/Ion channel Metabolism Calcium Channel Inhibitor inhibitor inhibit