keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
D-3, a phosphorpeptide, is an efficient, simple, and specific iPSC-eliminating agent.
产品描述 | D-3, a phosphorpeptide, is an efficient, simple, and specific iPSC-eliminating agent. |
体外活性 |
D-3 prevents residual iPSC-induced teratoma formation in a mouse tumorigenicity assay[1]. D-3 induces obvious loss of viability in 201B7 cells, with half maximal inhibitory concentration value of 192.3 ± 57.4 μM[1]. D-3 induces an increase in the amount of activated elF2a, p38, and p44/42 MAPK, which are activated in response to cellular stress. D-3 also increases the number of Annexin-V and SYTOX-positive cells, indicating apoptotic and dead cells after D-3 treatment[1]. D-3 has little influence on various non-iPSCs, including hepatocytes and neurons[1]. Cell Viability Assay[1]Cell Line: Six human iPSCs lines and one human ESC line (khES1). Concentration: 400 μM. Incubation Time: 1-2 h. Result: Sufficient to induce a viability loss (>99% in all iPSC lines and >95% in khES1). |
分子量 | 870.896 |
分子式 | C48H47N4O10P |
CAS No. | 1967815-98-0 |
keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
D-3 1967815-98-0 D3 D 3 Inhibitor inhibitor inhibit