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ACP5 Protein, Human, Recombinant (His)

ACP5 Protein, Human, Recombinant (His)

产品编号 TMPY-00960
别名: acid phosphatase 5, tartrate resistant, TRAP, SPENCDI

ACP5 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 35 kDa and the accession number is A0A024R7F8.

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ACP5 Protein, Human, Recombinant (His)
规格 价格/CNY 货期 数量
20 μg ¥ 3,170 5日内发货
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产品目录号及名称: ACP5 Protein, Human, Recombinant (His) (TMPY-00960)
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生物活性
技术参数
产品性质
参考文献
生物活性 Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is >100,000 pmol/min/μg.
产品描述 ACP5 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 35 kDa and the accession number is A0A024R7F8.
种属 Human
表达系统 HEK293 Cells
标签 C-His
蛋白编号 A0A024R7F8
别名 acid phosphatase 5, tartrate resistant, TRAP, SPENCDI
蛋白构建 Human ACP5 (NP_001602.1) precursor (Met 1-Pro 320)
蛋白纯度 > 95 % as determined by SDS-PAGE
分子量 35 kDa (predicted)
内毒素 < 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液 Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法 A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice.

研究背景 Tartrate-resistant acid phosphatase (TRACP) or acid phosphatase 5, tartrate resistant (ACP5 or TRAP) is a glycosylated monomeric metalloenzyme expressed in mammals. TRACP is associated with osteoblast migration to bone resorption sites, and, once there, TRACP is believed to initiate osteoblast differentiation, activation, and proliferation. TRACP once considered to be just a histochemical marker of osteoclasts is now recognised to be a molecule of widespread occurrence with functions in both the skeleton and the immune system. Two forms of TRACP circulate in human blood, TRACP 5a derived from macrophages and dendritic cells, and TRACP-5b derived from osteoclasts. Recent data have demonstrated the utility of TRACP-5b as a marker of osteoclast number and bone resorption, and serum TRACP-5a as a marker of inflammatory conditions. TRACP is expressed by osteoclasts, macrophages, dendritic cells and a number of other cell types. It has a critical role in many biological processes including skeletal development, collagen synthesis and degradation, the mineralisation of bone, cytokine production by macrophages and dendritic cells, macrophage recruitment, dendritic cell maturation and a role in the development of Th1 responses.

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Keywords

ACP5 Protein, Human, Recombinant (His) acid phosphatase 5, tartrate resistant TRAP SPENCDI recombinant recombinant-proteins proteins protein

 

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