规格

价格

库存

数量

5 μg

¥ 697

现货

10 μg

¥ 1,150

现货

20 μg

¥ 1,890

现货

50 μg

¥ 3,820

现货

100 μg

¥ 6,530

5日内发货

200 μg

¥ 11,100

5日内发货

500 μg

¥ 22,500

5日内发货

生物活性

产品描述	Alkaline phosphatase Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 54 kDa and the accession number is P09923.
生物活性	Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is > 1,000 pmoles/min/μg.
研究背景	ALPI encodes for intestinal phosphatase alkaline, a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.
种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	P09923
蛋白构建	A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1-Asp 503),without the pro peptide, was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Val 20
蛋白纯度	> 95 % as determined by SDS-PAGE
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
别名	IAP, alkaline phosphatase, intestinal
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	54 kDa (predicted); 66 kDa (reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

Alkaline phosphatase/ALPI Protein, Human, Recombinant (His)

Alkaline phosphatase/ALPI Protein, Human, Recombinant (His)

产品介绍

引用文献

计算器

剂量转换

技术支持