Antitumor agent-68 (Compound 7) shows anticancer activity with IC 50 s of 3.6 and 3.8 μM, for HeLa and MCF-7 cells, respectively [1]. Antitumor agent-68 (10 μΜ; 24 hours) leads to a 3-fold reduction of ROS production induced by Men in 3T3-L1 cells, and an EC 50 of 8.30 μg/mL for DPPH radical scavenging [1]. Cell Viability Assay [1] Cell Line: HeLa, MCF-7, 3T3-L1 Concentration: 10 μM Incubation Time: 24 hours Result: Showed anticancer activity by blocking the tubulin polymerization reaction. Reduced production of ROS, and scavenged DPPH radical.