Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Antioxidant agent-3 (Compound 14q) 是一种抗氧化剂,具有有效的DPPH 自由基清除活性和ABTS+清除活性,IC50分别为 26.58 和 30.31 μM。Antioxidant agent-3 作用于 H2O2处理的 HepG2 细胞,可增加活性氧 (ROS)、超氧化物歧化酶 (SOD) 和谷胱甘肽 (GSH),并减少乳酸脱氢酶(LDH)。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
25 mg | ¥ 10,600 | 10-14周 | ||
50 mg | ¥ 13,800 | 10-14周 | ||
100 mg | ¥ 17,500 | 10-14周 |
产品描述 | Antioxidant agent-3 (Compound 14q), a potent antioxidant, exhibits strong scavenging activity against DPPH radicals and ABTS+ radicals, with IC50 values of 26.58 μM and 30.31 μM, respectively. Additionally, it enhances the levels of reactive oxygen species (ROS), superoxide dismutase (SOD), and glutathione (GSH), while diminishing lactate dehydrogenase (LDH) in H2O2-treated HepG2 cells [1]. |
体外活性 | Antioxidant agent-3 (Compound 14q) exhibits good activity in DPPH radicals scavenging and ABTS + scavenging with the values of IC 50 is 26.58 μM and 30.31 μM respectively [1]. Antioxidant agent-3 shows low cytotoxicity in human normal WI-38 ( IC 50 > 100 μM) and GES ( IC 50 > 200 μM) cells [1]. Antioxidant agent-3 can enhance viability of H 2 O 2 -induced HepG2 cells [1]. Antioxidant agent-3 decreases the apoptotic percentage of HepG2 cells [1]. Antioxidant agent-3 reduces the ROS produce and LDH release, improves GSH and SOD levels in H 2 O 2 -treated HepG2 cells, and exhibits more stability in methanol solution [1]. Cell Cytotoxicity Assay [1] Cell Line: WI-38 and GES cells line Concentration: 200, 100, 50, 25 and 12.5 μM Incubation Time: 48 h Result: Showed less toxicity in hemolysis assay and weaker antiproliferative effects. Cell Viability Assay [1] Cell Line: H 2 O 2 -damaged WI-38 and HepG2 cells Concentration: 50, 25 and 12.5 μM Incubation Time: 1 h Result: Increased cells viability of H2O2-indcued cells and protected the H2O2-indcued cells against injury. Apoptosis Analysis [1] Cell Line: H 2 O 2 -damaged HepG2 cells Concentration: 50, 25 and 12.5 μM Incubation Time: 1 h Result: Protect the H 2 O 2 -injured HepG2 cells against apoptosis through antioxidant effect. |
分子量 | 358.3 |
分子式 | C18H14O8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Antioxidant agent-3 Inhibitor inhibitor inhibit