Anthraflavic acid (2,6-Dihydroxyanthraquinone), a flavonoid, is a potent IQ mutagenicity and cytochrome P-448 inhibitor.

The metabolic activation of IQ to mutagens was determined using the Ames mutagenicity test ?and employing Salmonella lyphimurium strain TA98 ?and 10% hepatic preparations as activation systems.?IQ and anthraflavic acid were dissolved in DMSO so that the solvent levels never exceeded 100 μl?per phile.?In experiments where microsomes onl~ were used as the activation system, $9 mix was supplemented with 1 unit/plate of glucose-6-phosphate dehydrogenase.?Pre-incubation conditions are described in the appropriate legends.