Streptozocin

规格	价格/CNY		货期	数量
100 mg	￥ 583	现货
500 mg	￥ 1,619	现货

规格

价格/CNY

货期

数量

100 mg

￥ 583

现货

500 mg

￥ 1,619

现货

存储

keep away from direct sunlight　|　Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

H2O: 113.3 mg/mL (427.19 mM), Sonication and heating are recommended.

DMSO: 30 mg/mL (113.11 mM), The compound is unstable in solution. Please use soon.

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
H2O / DMSO	1 mM	3.7705 mL	18.8523 mL	37.7045 mL	94.2614 mL
	5 mM	0.7541 mL	3.7705 mL	7.5409 mL	18.8523 mL
	10 mM	0.377 mL	1.8852 mL	3.7705 mL	9.4261 mL
	20 mM	0.1885 mL	0.9426 mL	1.8852 mL	4.7131 mL
	50 mM	0.0754 mL	0.377 mL	0.7541 mL	1.8852 mL
	100 mM	0.0377 mL	0.1885 mL	0.377 mL	0.9426 mL

产品描述	Streptozocin (NSC-85998) is an antibiotic that induces DNA methylation. Streptozocin is toxic to insulin-producing pancreatic islet B-cells and is commonly used in the construction of animal models of type 1 diabetes. The product is unstable in solution and is recommended to be dispensed now.
靶点活性	DNA methylating (C1498 cells):1024 μg/mL, DNA methylating (HL60 cells):11.7 μg/mL, DNA methylating (K562 cells):904 μg/mL
体外活性	方法：大鼠 β 胰岛素瘤细胞 Rin-5F 用 Streptozocin (1-10 mM) 处理 2-48 h，使用 MTT assay 检测细胞活力。结果：在用 10 mM Streptozocin 处理 24 h 和 48 h 的细胞中观察到最大的抑制 (60-70%)。[1] 方法：神经干细胞用 Streptozocin (2.5 mM) 处理 2 天，使用 RT-qPCR 检测基因表达。结果：Streptozocin 显著降低了 46.4% 的 GLUT3 mRNA 的相对表达水平，不影响 IR 和 GLUT1 的相对表达水平。[2]
体内活性	方法：将 Streptozocin (40 mg/kg in the 50 mM sodium citrate buffer (pH 4.5)，现配现用) 腹腔注射给 C57BL/6 或 CD-1 male 小鼠，每天一次，持续五天。给药期间提供正常食物和 10% 蔗糖水，第六天用普通水代替 10% 蔗糖水。结果：重复低剂量 Streptozocin 诱导小鼠 1 型糖尿病。[3] 方法：将 Streptozocin (200 mg/kg in the 50 mM sodium citrate buffer (pH 4.5)，现配现用) 单次腹腔注射给 C57BL/6 或 CD-1 male 小鼠。给药后提供正常食物和 10% 蔗糖水，第三天用普通水代替 10% 蔗糖水。结果：单次高剂量 Streptozocin 诱导小鼠 1 型糖尿病。[3]
细胞实验	Streptozocin (STZ) stock solutions of 50 mg/mL in distilled Water (dWater) are freshly prepared for each experiment[2]. Human and murine cell lines are cultured in triplicate in 96-well plates at a density of 2×104 cells/well in the absence (untreated control) or presence of various concentrations of ALX (20-3000 μg/mL) or STZ (1-3000 μg/mL) for 48 h at 37°C under a humidified atmosphere containing 5% CO2. Cells incubated in complete media including dWater in a final concentration of 0.1% served as control for solvent toxicity and cells incubated in complete medium are used as a control for the experiments. The effects of the tested drugs on tumor cell growth or viability are determined employing the MTT assay in accordance with the manufacturer's instructions. The IC50values (drug concentration that induces 50% inhibition of the cell growth) are calculated using the GraphPad Prism 4 program[2].

别名	STZ, Streptozotocin, 链脲佐菌素, NSC-85998, 链脲菌素, U 9889
分子量	265.22
分子式	C8H15N3O7
CAS No.	18883-66-4

存储

溶解度

溶液配制表

计算器

输入分子式，点击计算，可计算出产品的分子量。

参考文献

文献引用

相关产品

相关化合物库

剂量换算

体内实验配液计算器

技术支持

Keywords