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NNMTi

产品编号 T8760 CAS 42464-96-0

别名: 5-Amino-1-methylquinolinium

NNMTi (5-Amino-1-methylquinolinium) 是烟酰胺 N -甲基转移酶的抑制剂 (IC50=1.2 μM)，可选择性地结合 NNMT 底物结合位点残基。它能诱导体外成肌细胞分化，提高老年小鼠肌肉干细胞融合和再生能力。

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NNMTi, CAS 42464-96-0

规格	价格/CNY	货期
2 mg	￥ 297	现货
5 mg	￥ 493	现货
10 mg	￥ 758	现货
25 mg	￥ 1,430	现货
50 mg	￥ 2,380	现货
100 mg	￥ 3,550	现货
1 mL * 10 mM (in DMSO)	￥ 517	现货

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选择批次

纯度: 99.93%

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生物活性

化学信息

存储 & 溶解度

参考文献

产品描述

NNMTi (5-Amino-1-methylquinolinium) is a substrate site-targeting, selective nicotinamide N-methyltransferase (NNMT) inhibitor (IC50 = 1.2 μM; 50 μM SAM, 100 μM NCA) that reduces 3T3-L1 lipogenesis (EC50 = 30μM) and adipocytes 1-methylnicotinamide level (EC50 = 2.3 μM) without affecting related methyltransferases & enzymes in the NAD+ salvage pathway

体内活性

NNMTi treatment will rescue age-related deficits in muSC activity to promote superior regeneration post-injury in aging muscle.?24-month old mice were treated with saline (control), and low and high dose NNMTi (5 and 10 mg/kg) for 1-week post-injury, or control and high dose NNMTi for 3-weeks post-injury.?All mice underwent an acute muscle injury (barium chloride injection) locally to the tibialis anterior (TA) muscle, and received 5-ethynyl-2'-deoxyuridine systemically to analyze muSC activity.?In vivo contractile function measurements were conducted on the injured TA muscle and tissues collected for ex-vivo analyses, including myofiber cross-sectional area (CSA) measurements to assess muscle recovery.?Results revealed that muscle stem cell proliferation and subsequent fusion were elevated in NNMTi-treated mice, supporting nearly 2-fold greater CSA and shifts in fiber size distribution to greater proportions of larger sized myofibers and fewer smaller sized fibers in NNMTi-treated mice compared to controls.?Prolonged NNMTi treatment post-injury further augmented myofiber regeneration evinced by increasingly larger fiber CSA.?Importantly, improved muSC activity translated not only to larger myofibers after injury but also to greater contractile function, with the peak torque of the TA increased by 70% in NNMTi-treated mice compared to controls.?Similar results were recapitulated in vitro with C2C12 myoblasts, where NNMTi treatment promoted and enhanced myoblast differentiation with supporting changes in the cellular NAD+/NADH redox states[1].

别名	5-Amino-1-methylquinolinium
分子量	286.11
分子式	C10H11IN2
CAS No.	42464-96-0

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 2.86 mg/mL (10.01 mM)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.4952 mL	17.4758 mL	34.9516 mL	87.379 mL
	5 mM	0.699 mL	3.4952 mL	6.9903 mL	17.4758 mL
	10 mM	0.3495 mL	1.7476 mL	3.4952 mL	8.7379 mL

计算器

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参考文献

1. Harshini Neelakantan, et al. Small molecule nicotinamide N-methyltransferase inhibitor activates senescent muscle stem cells and improves regenerative capacity of aged skeletal muscle. Biochem Pharmacol. 2019 May;163:481-492. 2. Benjamin Trinité, Hongtao Zhang, David N Levy ,et al.NNRTI-induced HIV-1 protease-mediated cytotoxicity induces rapid death of CD4 T cells during productive infection and latency reversal.Retrovirology. 2019 Jun 26;16(1):17.

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

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第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

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