规格

价格

库存

数量

100 mg

¥ 150

现货

1 mL x 10 mM (in DMSO)

¥ 99

现货

生物活性

产品描述	3,4-Dihydroxybenzylamine hydrobromide (NSC-263475 hydrobromide) inhibits DNA polymerase activity in melanoma cells and displays growth inhibitory activity in melanoma cell lines with varying degrees of tyrosinase activity.
体外活性	3,4-Dihydroxybenzylamine hydrobromide (1 μM-10 mM; 48 hours) inhibits the growth of all melanoma cell lines (IC50 = 100 μM) and the SK-MELB cell line (IC50 = 122 μM). The IC50 values are 30, 84,90, and 68 μM for Human SK-MEL-30, SK-MEL-2, SK-MEL-3,RPMI-7951 cells, respectively. 3,4-Dihydroxybenzylamine hydrobromide (1 μM-10 mM; 48 hours) cytotoxicity in SK-MEL-2 and SK-MEL-28 cell lines can be enhanced by application of buthionine sulfoximine (BSO). BSO treatment sensitizes marginally pigmented cells to the effects of 3,4-DHBA, and the presence of decreses IC50 values of 3,4-DHBA by 127% in the SK-MEL-28 cell line and by 148% in the SK-MEL-2 cell line. 3,4-Dihydroxybenzylamine hydrobromide exhibits IC50values of 10, 25, 67, and 184 μM for S91A, S91B, L1210, and SCC-25 cells, respectively[1].
体内活性	3,4-Dihydroxybenzylamine hydrobromide (200, 400, 600 and 800 mg/kg; i.p.; 21 days) has different effects at different doses, the median life-span are 17, 24.5, 26, 29 and 25 days respectively. 3,4-Dihydroxybenzylamine hydrobromide (1000 mg/kg; i.p.; 7 days) has the least toxic effect in non-tumor-bearing B6D2F1 mice,and mice is tolerated at this dose[2].
细胞实验	Cell Line: Human and Murine Melanoma Cell Lines; Non-Melanoma Cell Lines Concentration: 1 μM-10 mM Incubation Time: 48 hours [1]
动物实验	Animal Model: C57BL/6 mice with B6D2F1 cells Dosage: 200 mg/kg, 400 mg/kg, 600 mg/kg, and 800 mg/kg Administration: Intraperitoneal injection [2]
别名	NSC 263475 hydrobromide, 3,4-二羟基苄胺·氢溴酸

化学信息

分子量	220.06
分子式	C₇H₁₀BrNO₂
CAS No.	16290-26-9
Smiles	Br.NCc1ccc(O)c(O)c1
密度	1.309g/cm3

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

实际储存温度请以COA为准

溶解度信息

DMSO: 80 mg/mL (363.54 mM), Sonication is recommended.

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (15 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.5442 mL	22.7211 mL	45.4422 mL	227.2108 mL
5 mM	0.9088 mL	4.5442 mL	9.0884 mL	45.4422 mL
10 mM	0.4544 mL	2.2721 mL	4.5442 mL	22.7211 mL
20 mM	0.2272 mL	1.1361 mL	2.2721 mL	11.3605 mL
50 mM	0.0909 mL	0.4544 mL	0.9088 mL	4.5442 mL
100 mM	0.0454 mL	0.2272 mL	0.4544 mL	2.2721 mL

该溶液配制表仅适用于固体产品。对于液体产品，请根据标明的浓度或密度计算稀释方案。

3,4-Dihydroxybenzylamine hydrobromide

3,4-Dihydroxybenzylamine hydrobromide

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词

3,4-Dihydroxybenzylamine hydrobromide

3,4-Dihydroxybenzylamine hydrobromide