Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Nrf2/HO-1 activator 2 (compound 13m), a difluoro-substituted derivative, is a highly potent activator of Nrf2/HO-1. It exhibits substantial neuroprotective and antioxidant properties by activating the Nrf2/HO-1 pathway through phosphorylation of ERK1/2, JNK, or Akt in PC12 cells. This compound finds utility in the investigation of Parkinson's disease (PD) [1].
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
25 mg | ¥ 10,600 | 10-14周 | ||
50 mg | ¥ 13,800 | 10-14周 | ||
100 mg | ¥ 17,500 | 10-14周 |
产品描述 | Nrf2/HO-1 activator 2 (compound 13m), a difluoro-substituted derivative, is a highly potent activator of Nrf2/HO-1. It exhibits substantial neuroprotective and antioxidant properties by activating the Nrf2/HO-1 pathway through phosphorylation of ERK1/2, JNK, or Akt in PC12 cells. This compound finds utility in the investigation of Parkinson's disease (PD) [1]. |
体外活性 | Nrf2/HO-1 activator 2 (compound 13m; 0.1-30 μM; 24 h) has protection to PC12 cells against toxicity and inhibits 6-OHDA- and Rotenone - induced cell death [1]. Nrf2/HO-1 activator 2 (1-100 μM; 11 h) inhibits 6-OHDA- or rotenone-induced production of reactive oxygen species and partially attenuates lipid peroxidation in rat brain homogenates. Nrf2/HO-1 activator 2 inhibits the production of lipid peroxide in rat brain homogenates by 28.8% at 100 μM [1]. Nrf2/HO-1 activator 2 (1-100 μM; 11 h) up-regulates heme oxygenase-1 (HO-1) and Nrf2 expression [1]. Nrf2/HO-1 activator 2 (10 μM; 2-3 h; PC12 cells) activator 2 activates Nrf2/HO-1 signaling by phosphorylated ERK1/2, JNK, and Akt [1]. Cell Viability Assay [1] Cell Line: PC12 cells Concentration: 3, 5, 7, 10 and 30 μM, 6-OHDA (50 μM); 0.1, 0.3, 1, 3, 10, and 30 μM, Rotenone (1 μM) Incubation Time: 24 hours Result: Exhibited neuroprotective effects on 6-OHDA-induced toxicity in dose-dependent manners. Rescued cells from rotenone-induced damage, with increased levels of cell viability up to 79.9% at 30 μM. Western Blot Analysis [1] Cell Line: PC12 cells Concentration: 0, 1, 3, 10, and 30 μM Incubation Time: 24 hours Result: Upregulated HO-1 levels in a dose-dependent manner, with 1.5- and 2-fold increases at 30 μM concentrations, respectively. Enhanced the nuclear translocation of Nrf2 in PC12 cells after 3 h at 10 μM. Western Blot Analysis [1] Cell Line: PC12 cells Concentration: 10 μM Incubation Time: 2-3 hours Result: Induced the phosphorylation of ERK1/2, JNK, and Akt. |
分子量 | 374.33 |
分子式 | C20H16F2O5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Nrf2/HO-1 activator 2 Inhibitor inhibitor inhibit