Name: NLRP3-IN-32
Brand: TargetMol
SKU: T209380
Rating: 4.5 (1 reviews)

规格

价格

库存

数量

10 mg

待询

50 mg

待询

产品介绍

生物活性

产品描述

NLRP3-IN-32 (compound 7a) is a derivative of 3,4-dihydronaphthalene-1(2H)-one and acts as an inhibitor of the NLRP3 inflammasome. It obstructs the assembly and activation of the NLRP3 inflammasome by downregulating the expression of NLRP3 and the apoptosis-associated speck-like protein containing a CARD (ASC), thereby reducing the production of reactive oxygen species (ROS) and other inflammatory mediators. Additionally, NLRP3-IN-32 inhibits the phosphorylation of IκBα and NF-κB/p65, as well as the nuclear translocation of p65, thus suppressing NF-κB signaling.

体外活性

NLRP3-IN-32（compound 7a；1.5、3、6 μM）通过剂量依赖性方式逆转LPS刺激的RAW246.7巨噬细胞中炎症因子TNF-α、IL-6、IL-18和IL-1β的释放。该化合物在相同浓度下有效清除细胞内由LPS刺激产生的ROS和NO，并抑制NLRP3炎症小体的激活。NLRP3-IN-32对RAW246.7细胞表现出低毒性，不同剂量下细胞凋亡率分别为3.8%、5.6%和6.8%。此外，NLRP3-IN-32（1.5、3、6 μM；24小时）能够剂量依赖性地抑制LPS（1.0 μg/mL；2小时）刺激的IκBα磷酸化和p65磷酸化，进而抑制NF-κB信号通路的激活。