Name: CDDO-Im
Brand: TargetMol
SKU: TQ0120
Price: 297 CNY
Availability: InStock
Rating: 5 (10 reviews)

规格

价格

库存

数量

1 mg

¥ 297

现货

5 mg

¥ 597

现货

10 mg

¥ 928

现货

25 mg

¥ 1,620

现货

50 mg

¥ 2,620

现货

100 mg

¥ 3,970

现货

500 mg

¥ 8,630

现货

1 mL x 10 mM (in DMSO)

¥ 712

现货

产品介绍

生物活性

产品描述	CDDO-Im (TP-235) is an activator of Nrf2 and PPAR (Kis: 232/344 nM for PPARα/PPARγ).
靶点活性	PPARγ:344 nM (Ki), PPARα:232 nM (Ki)
体外活性	CDDO-Im在抑制人类白血病和乳腺癌细胞系的细胞增殖方面具有高活性（IC50约为10-30 nM）。在U937白血病细胞中，通过增加CD11b和CD36细胞表面表达来衡量，CDDO-Im还诱导单核细胞分化[1]。CDDO-Im处理在三阴性乳腺癌细胞系SUM159和MDA-MB-231中明显诱导细胞周期在G2/M期停滞和凋亡。在SUM159肿瘤球中的CD24^-/EpCAM^+细胞显著被CDDO-Im处理抑制。CDDO-Im还显著降低了初级和二级球体培养中的球体形成效率和肿瘤球大小[2]。用CDDO-Im处理能提高Nrf2蛋白水平，Nrf2是一种先前显示能结合ARE序列并增加Nrf2调控的多种抗氧化和解毒基因表达的转录因子[3]。
体内活性	CDDO-Im 对初级小鼠巨噬细胞中新生可诱导型一氧化氮合酶(iNOS)表达具有显著的抑制作用。此外，CDDO-Im 在体内抑制B16小鼠黑色素瘤和L1210小鼠白血病细胞的生长。CDDO-Im 10 nM（5.4 μg）的注射几乎完全阻止了IFN-γ诱导iNOS的能力，而使用少至1 nmol的CDDO-Im（0.54 μg）治疗也具有部分效果[1]。
细胞实验	CDDO-Im is dissolved in DMSO. SUM159 and MDA-MB-231 cells are seeded into each well of 96-well plates (1,000 cell/well) and treated the next day with vehicle control or CDDO-Im (1, 10, 50, 100 and 200 nM) for given incubation time. The absorbance is measured with a spectrophotometer to determine the cell proliferation rate [2].
动物实验	Mice are injected i.p. with thioglycollate, and the resulting resident peritoneal macrophages are activated 3 days later with an i.p. injection of IFN-γ. CDDO and CDDO-Im are injected i.p. 30 min after IFN-γ. Macrophages are harvested 10 h later, cultured for 12 h, and then assayed for expression of iNOS protein and production of nitric oxide (NO) [1].
别名	TP-235, RTA-403, CDDO-Imidazolide

化学信息

分子量	541.72
分子式	C₃₄H₄₃N₃O₃
CAS No.	443104-02-7
Smiles	[H][C@@]12CC(C)(C)CC[C@@]1(CC[C@]1(C)[C@]2([H])C(=O)C=C2[C@@]1(C)CC[C@@]1([H])C(C)(C)C(=O)C(=C[C@]21C)C#N)C(=O)n1ccnc1
密度	1.23 g/cm3 (Predicted)
颜色	White
物理性状	Solid

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

溶解度信息

DMSO: 40 mg/mL (73.84 mM), Sonication is recommended.

H2O: Insoluble

溶液配制表

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.8460 mL	9.2299 mL	18.4597 mL	92.2986 mL
5 mM	0.3692 mL	1.8460 mL	3.6919 mL	18.4597 mL
10 mM	0.1846 mL	0.9230 mL	1.8460 mL	9.2299 mL
20 mM	0.0923 mL	0.4615 mL	0.9230 mL	4.6149 mL
50 mM	0.0369 mL	0.1846 mL	0.3692 mL	1.8460 mL

体内实验配液计算器