PCSK9 重组蛋白, Mouse (mFc)

PCSK9 Protein, Mouse, Recombinant (mFc)

产品编号 TMPY-05217

PCSK9 Protein, Mouse, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 97.6 kDa and the accession number is Q80W65.

规格	价格	库存	数量
20 μg	¥ 1,630	5日内发货
100 μg	¥ 3,820	5日内发货

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TargetMol 的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。

常见问题解答

问重组蛋白产品该如何保存？

答1) 存储条件:重组蛋白产品收到后，如不立即使用，应当保存在 -20℃ 至 -80℃ 的条件下。冻干粉可稳定保存 12 个月以上，液体类产品在 -80℃ 条件下可保存 6-12 个月。已复溶的重组蛋白样品需尽快用完，如果不能在一周内用完，需在 -20℃ 至 -80℃ 保存，可保存 3 个月以上\n2) 避免反复融冻:重组蛋白产品应注意避免反复冻融，建议将重组蛋白产品分装成多份。分装冻存时，建议选择含有一定浓度载体蛋白的溶液或培养基复溶产品。产品浓度不低于 100 ug/mL，且每管的体积不小于 20 uL。

问如何将冻干的重组蛋白配置为储备液？

答在打开盖⼦之前，先使用 10000-12000 rpm 的转速进行离心，20-30 秒，将附着在管盖或管壁上的冻⼲粉收集到管底，以避免损失。大多数蛋白可通过加入无菌蒸馏水来重新溶解。如果需要除水以外的其他稀释剂，产品 COA 中会对其进行说明。一般储备液浓度建议不要低于 100 μg/mL，分装储存避免反复冻融。

问蛋白能保证活性吗？

答我们有现货的产品，有活性数据的话会展示在官网生物活性那栏，没有数据的也不代表就一定没有活性，是我们还没有建立活性检测方法，没有进行测试，但是我们的蛋白都是优先按照有活性的方法去表达纯化的。

问重组蛋白有吗？

答您需要什么种属的蛋白呢，对表达系统和标签有要求吗，用于什么实验？

问为什么重组蛋白的体积和质量与预期不符？

答蛋白冻干粉由蛋白原液冻干而来，冻干粉质量不等同于蛋白质量。由于冻⼲产品的体积大⼩受多种因素影响，包括冻⼲前缓冲液的组分、盐离⼦浓度以及产品本身的浓度等。冻⼲粉可能看上去很多，也可能肉眼不易观察到，这些都是正常现象，请您放心使用。在收到产品后，照常复溶、使用或分装储存即可。

产品信息

生物活性	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述	PCSK9 Protein, Mouse, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 97.6 kDa and the accession number is Q80W65.
种属	Mouse
表达系统	HEK293 Cells
标签	C-mFc
蛋白编号	Q80W65
别名	proprotein convertase subtilisin/kexin type 9,PC9,Narc1,HCHOLA3,FH3,AI747682,AI415265
蛋白构建	A DNA sequence encoding the mouse PCSK9 (NP_705793.1) (Met1-Gln694) was expressed with the Fc region of mouse IgG1 at the C-terminus. Predicted N terminal: Gln 35
蛋白纯度	> 95 % as determined by SDS-PAGE.
分子量	97.6 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

PCSK9 Protein, Mouse, Recombinant (mFc)

PCSK9 Protein, Mouse, Recombinant (mFc)

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