keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Z-LEHD-FMK TFA is a specific and irreversible inhibitor of caspase-9, offering protection against detrimental reperfusion injury and moderating apoptosis. Additionally, Z-LEHD-FMK TFA demonstrates its neuroprotective potential in a rat model of spinal cord trauma.
产品描述 | Z-LEHD-FMK TFA is a specific and irreversible inhibitor of caspase-9, offering protection against detrimental reperfusion injury and moderating apoptosis. Additionally, Z-LEHD-FMK TFA demonstrates its neuroprotective potential in a rat model of spinal cord trauma. |
体外活性 | Z-LEHD-FMK (20 μM; pretreated for 30 min) completely protects HCT116 and 293 cells from TRAIL-induced toxicity[1]. Z-LEHD-FMK (20 μM ; 6 h) protects normal human hepatocytes from TRAIL-induced apoptosis[1]. Apoptosis Analysis[1]Cell Line: SW480, H460, HCT116 and 293 cells Concentration: 20 μM Incubation Time: Pretreated for 30 min Result: Protected HCT116 and 293 cells from TRAIL-induced apoptosis. Western Blot Analysis[1]Cell Line: HCT116, SW480 cells Concentration: 20 μM Incubation Time: 2 h Result: Protected procaspase 3 from cleavage in HCT116 cells but not in SW480 cells, especially at the 16-h time point. |
体内活性 | Z-LEHD-FMK (0.8 μmol/kg; i.v. for 7 d) protects neurons, glia, myelin, axons, and intracellular organelles in spinal cord injury (SCI) rats[2]. Animal Model: Male Wistar albino rats (250-350 g) with SCI[2]Dosage: 0.8 μmol/kg Administration: I.v. for 1 or 7 days Result: Decreased the mean apoptotic cell count at 24 hours and 7 days postinjury. |
分子量 | 804.75 |
分子式 | C34H44F4N6O12 |
CAS No. | 524746-03-0 |
keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Z-LEHD-FMK TFA 524746-03-0 ZLEHDFMK TFA Z LEHD FMK TFA Inhibitor inhibitor inhibit