SPC-180002, a dual SIRT1/3 inhibitor, exhibits IC50 values of 1.13 and 5.41 μM for SIRT1 and SIRT3, respectively. It disrupts redox homeostasis through ROS generation, consequently enhancing p21 protein stability and inducing mitochondrial dysfunction. Furthermore, this compound significantly impedes cell cycle progression and malignancy proliferation, while also activating the Nrf2 signaling pathway [1].

SIRT1:1.13 μM, SIRT3:5.41 μM

SPC-180002（0-5 μM，24小时）能够通过积累p21来阻滞细胞周期，进而引发细胞衰老，但不会诱发细胞凋亡或自噬[1]。

SPC-180002（1-5mg/kg，周两次腹腔注射）能有效抑制多癌细胞增殖，并阻止MCF7肿瘤移植小鼠肿瘤生长[1]。