keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
PROTAC-O4I2 是一种靶向剪接因子 3B1 (SF3B1) 的 PROTAC连接体, 在 K562 细胞中促使 FLAG-SF3B1 降解,IC50 值为 0.244 μM。PROTAC-O4I2 促使 K562 WT 细胞凋亡 (apoptosis)。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 543 | 现货 | ||
5 mg | ¥ 1,330 | 现货 | ||
10 mg | ¥ 1,980 | 现货 | ||
25 mg | ¥ 4,570 | 现货 | ||
50 mg | ¥ 7,890 | 现货 | ||
100 mg | ¥ 10,600 | 现货 | ||
500 mg | ¥ 21,300 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,460 | 现货 |
产品描述 | PROTAC-O4I2, a PROTAC ligand targeting splicing factor 3B1 (SF3B1), induced FLAG-SF3B1 degradation in K562 cells with an IC50 value of 0.244 μM. PROTAC-O4I2 induced apoptosis in K562 WT cells. |
体外活性 |
PROTAC-O4I2 introduces thalidomide into the ubiquitin E3 ligase cereblon (CRBN), thereby selectively degrading SF3B1 and inhibiting tumor growth in cells.[1] PROTAC-O4I2 degrades and inhibits SF3B1 in K562 cells. PROTAC-O4I2 has anti-proliferative effects on SF3B1 WT, SF3B1 OE, and SF3B1 K700E cells with IC50 of 228, 63, and 90 nM, respectively.[1] PROTAC-O4I2 induces FLAG-SF3B1 degradation in a concentration-dependent manner, with a half-maximal inhibitory concentration (IC50) value of 0.244 μM in K562 cells.[1] |
体内活性 | Flies were fed on a round filter paper loaded with PROTAC-O4I2 (10 μM) in a 5% sucrose solution, maintained at 18℃, and flipped into a freshly prepared vial every 2 days. PROTAC-O4I2 significantly improves Drosophila survival in a Drosophila intestinal tumor model by interfering with tumor maintenance and proliferation.[1] |
分子量 | 609.1 |
分子式 | C29H29ClN6O5S |
CAS No. | 2785323-62-6 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 200 mg/mL (328.35 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.6418 mL | 8.2088 mL | 16.4177 mL | 41.0442 mL |
5 mM | 0.3284 mL | 1.6418 mL | 3.2835 mL | 8.2088 mL | |
10 mM | 0.1642 mL | 0.8209 mL | 1.6418 mL | 4.1044 mL | |
20 mM | 0.0821 mL | 0.4104 mL | 0.8209 mL | 2.0522 mL | |
50 mM | 0.0328 mL | 0.1642 mL | 0.3284 mL | 0.8209 mL | |
100 mM | 0.0164 mL | 0.0821 mL | 0.1642 mL | 0.4104 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
PROTAC-O4I2 2785323-62-6 Apoptosis PROTAC PROTACs Inhibitor inhibitor inhibit