Phenazine-1-carboxylic acid exhibits a broad spectrum of bioactivities including antibacterial effects against Bacillus cereus (MIC < 0.5 µg/mL), Micrococcus luteus (MIC < 5.0 µg/mL), and Staphylococcus aureus (MIC < 5.0 µg/mL) observed through disk assay over 2 days. It also demonstrates antibacterial activity against methicillin-resistant Staphylococcus aureus, Mycobacterium fortuitum, Mycobacterium aurum, Mycobacterium vaccae, and Mycobacterium smegmatis with MIC values ranging from 25.0 to 100.0 µg/mL. Additionally, it inhibits Mycobacterium tuberculosis H37Rv ATCC 27292 with a MIC90 of 32.9 µg/mL, as determined using the Alamar blue assay. The compound displays substantial antifungal properties, inhibiting the mycelial growth of various fungal strains such as Botryosphaeria berengeriana, Fusarium spp., Mycosphaerella arachidis, Alternaria solani, and Colletotrichum falcatum, with EC50 values ranging from 3.58 to 18.5 µg/mL and an inhibition zone of 2.0 mm at 100 µg/disc after 10 to 15 days. Furthermore, Phenazine-1-carboxylic acid shows diverse bioactivities in multiple assays, including inhibition of ALDH1A1, influenza NS1 protein function, TGF-β, and inducing DNA re-replication in colon adenocarcinoma cells. However, it has no significant activity against human AChE, Bacillus subtilis DSM 347, or KIF5 cells, with IC50 values greater than 50000 nM. It also exhibits cytotoxicity against HCT116 cells with an IC50 value of 15600 nM after 5 days..
Note: Summary generated by AI. Data source: ChEMBL 