Powder: -20°C for 3 years | In solvent: -80°C for 1 year
JH-X-119-01 hydrochloride is a potent and selective inhibitor of interleukin-1 receptor-associated kinases 1 (IRAK1). It effectively alleviates lipopolysaccharide (LPS)-induced sepsis in mice.
产品描述 | JH-X-119-01 hydrochloride is a potent and selective inhibitor of interleukin-1 receptor-associated kinases 1 (IRAK1). It effectively alleviates lipopolysaccharide (LPS)-induced sepsis in mice. |
体外活性 | Jh-X-119-01 (10 μM) decreases phosphorylation of NF-κB and mRNA levels of IL-6 and TNFα in LPS-treated macrophages in vitro. Jh-X-119-01 selectively inhibits IRAK1 phosphorylation[1]. Western Blot Analysis[1]Cell Line: RAW 264.7 cells and THP-1 cells Concentration: 10 μM Incubation Time: 15 minutes Result: Decreased LPS (100 ng/mL)-induced phosphorylation of IκBα and NF-κB-P65. |
体内活性 | Jh-X-119-01 improves survival and decreases immunopathies of LPS-challenged mice. Jh-X-119-01 increases survival of mice at the dose of 5 mg/kg body weight. Survival is further improved when the dose is increased to 10 mg/kg[1]. Animal Model: C57BL/6 (20-22 g, male) mice[1]Dosage: 5 mg/kg and 10 mg/kg Administration: Intraperitoneally injected; 5 days Result: Protected mice from LPS (20 mg/kg)-induced sepsis. Survival at day 5 was 13.3% in control group where septic mice were treated by vehicle, while the values were 37.5% and 56.3% for 5 mg/kg and 10 mg/kg. |
分子量 | 488.93 |
分子式 | C25H21ClN6O3 |
CAS No. | 2591344-30-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
JH-X-119-01 hydrochloride 2591344-30-6 JH-X-119-01 Hydrochloride JHX11901 hydrochloride JH X 119 01 hydrochloride Inhibitor inhibitor inhibit