Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Hcyb1 is a potent and specific inhibitor of phosphodiesterase 2 (PDE2). It exhibits a high degree of selectivity for inhibiting PDE2A with an IC50 value of 0.57 μM. In addition, Hcyb1 displays over 250-fold selectivity against other recombinant members of the PDE family. Moreover, its pharmacological actions include neuroprotective and antidepressant-like effects, which are believed to be mediated through the cAMP/cGMP-CREB-BDNF signaling pathway [1].
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
25 mg | ¥ 9,160 | 10-14周 | ||
50 mg | ¥ 11,900 | 10-14周 | ||
100 mg | ¥ 17,634 | 10-14周 |
产品描述 | Hcyb1 is a potent and specific inhibitor of phosphodiesterase 2 (PDE2). It exhibits a high degree of selectivity for inhibiting PDE2A with an IC50 value of 0.57 μM. In addition, Hcyb1 displays over 250-fold selectivity against other recombinant members of the PDE family. Moreover, its pharmacological actions include neuroprotective and antidepressant-like effects, which are believed to be mediated through the cAMP/cGMP-CREB-BDNF signaling pathway [1]. |
体外活性 | Hcyb1 increases cGMP levels by 1.7~2.3 folds after 10-minute treatment at concentrations of 1~100 nM [1]. Hcyb1 increases both cGMP and cAMP levels 24 hours after treatment at the concentrations of 1 nM [1]. Hcyb1 treatment also increases the levels of phosphorylation of CREB and BDNF in HT-22 cells for 24 hours [1]. Hcyb1 promotes HT-22 cell viability and increase the cGMP and cAMP accumulation in HT-22 cells [1]. Hcyb1 exhibits the concentration- and time-dependent effects on cell viability in HT-22 cells [2]. Cell Proliferation Assay [1] Cell Line: HT-22 cells Concentration: 1 pM, 0.01 nM, 0.1 nM, 1 nM, 0.01 μM, 0.1 μM, 1 μM, 10 μM Incubation Time: 24 hours Result: The cell viability was significantly increased when treatment HT-22 cells with Hcyb1 at concentrations of 0.1 nM and 1 nM for 24 hours. The time-dependent effects showed that the cell viability was significantly increased from 12 to 24 hours when treatment at concentration of 1 nM. The maximal effects peaked at 24 hours after treatment. Western Blot Analysis [1] Cell Line: HT-22 cells Concentration: 1 nM Incubation Time: 24 hours Result: Induced a significant increase in the phosphorylation of CREB. BDNF expression was also significantly upregulated at the same concentration. |
体内活性 | Hcyb1 (0.5, 1, and 2 mg/kg, i.g.) decreases the immobility time in both forced swimming and tail suspension tests, without altering locomotor activity [3]. Animal Model: Male imprinting control region (ICR) mice, weighing between 20 and 25 g [3] Dosage: 0.5, 1, and 2 mg/kg Administration: Gavage (i.g.) Result: Exhibited dose-dependent reduction in immobility time at doses of 0.5, 1, 2 mg/kg (i.g.). |
分子量 | 380.44 |
分子式 | C24H20N4O |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Hcyb1 Inhibitor inhibitor inhibit