EHT 5372 is a potent and selective inhibitor of DYRK’s family kinases. EHT 5372 shows IC 50 s of 0.22, 0.28, 10.8, 93.2, 22.8, 88.8, 59.0, 7.44, 221 nM for DYRK1A, DYRK1B, DYRK2， DYRK3， CLK1, CLK2, CLK4, GSK-3α, GSK-3β.

EHT 5372 (0.1-10 μM; 24 hours) dose-dependently reduces pS396-Tau levels with an IC 50 of 1.7 μM whereas cell viability remains over 87% in all conditions [1]. EHT 5372 (0.01-1 μM) inhibits the direct phosphorylation of Tau by DYRK1A [1] EHT 5372 reduces Aβ production in a dose-dependent reduction with an IC 50 of 1.06 μM [1]. Cell Viability Assay [1] Cell Line: HEK293 cells Concentration: 0.1, 0.5, 1, 5, 10 μM Incubation Time: 24 hours Result: Cell viability remained over 87% in all conditions. Western Blot Analysis [1] Cell Line: HEK293 cells Concentration: 0.01, 0.03, 0.1, 0.3, 1 μM Incubation Time: Result: Potently and dose-dependently inhibited Tau phosphorylation at pS396.