Cys-PKHB1 (Cys-txCD47) is a polypeptide consisting of a cysteine (Cys) residue coupled with PKHB1, which is a CD47 agonist peptide that acts as a thrombospondin-1 peptide mimetic with anti-tumor properties. In breast cancer cells, PKHB1 induces mitochondrial changes, ROS production, intracellular Ca+ accumulation, and calcium-dependent cell death. Additionally, PKHB1 activates the immune system by inducing immunogenic cell death in breast cancer cells.