Powder: -20°C for 3 years | In solvent: -80°C for 1 year
ART558 是一种高效的、选择性的、变构 DNA 聚合酶活性的 Polθ 抑制剂,IC50为7.9 nM。ART558 可用于癌症研究。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 1,220 | 现货 | ||
5 mg | ¥ 3,130 | 现货 | ||
10 mg | ¥ 4,590 | 现货 | ||
25 mg | ¥ 7,320 | 现货 | ||
50 mg | ¥ 9,860 | 现货 | ||
100 mg | ¥ 13,300 | 现货 |
产品描述 | ART558 is a potent, selective, low molecular weight, allosteric DNA polymerase activity of Polθ inhibitor (IC50=7.9 nM). ART558 can be used for the research of cancer. |
体外活性 | ART558 (5μM; 0~72 hours; BRCA2wild-type or BRCA2‒/‒ cells) shows γH2AX accumulation in cells. ART558 (0~10 μM; 7 days; BRCA2wild-type or BRCA2‒/‒ cells) shows synthetic lethality and a combinatorial effect with the PARPi olaparib in previously described isogenic models of BRCA1-deficiency. ART558 inhibits the major Polθ mediated DNA repair process, Theta-Mediated End Joining, without targeting NonHomologous End Joining. ART558 elicits DNA damage and synthetic lethality in BRCA1- or BRCA2-mutant tumour cells and enhances the effects of a PARP inhibitor. ART558 increases biomarkers of single-stranded DNA and synthetic lethality in 53BP1-defective cells whilst the inhibition of DNA nucleases that promote end-resection reversed these effects, implicating these in the synthetic lethal mechanism-of-action. ART558 increases the residence time of YFP-tagged full-length Polθ at sites of laserinduced DNA damage[1]. |
分子量 | 418.41 |
分子式 | C21H21F3N4O2 |
CAS No. | 2603528-97-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 150 mg/mL (358.5 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.39 mL | 11.95 mL | 23.9 mL | 59.75 mL |
5 mM | 0.478 mL | 2.39 mL | 4.78 mL | 11.95 mL | |
10 mM | 0.239 mL | 1.195 mL | 2.39 mL | 5.975 mL | |
20 mM | 0.1195 mL | 0.5975 mL | 1.195 mL | 2.9875 mL | |
50 mM | 0.0478 mL | 0.239 mL | 0.478 mL | 1.195 mL | |
100 mM | 0.0239 mL | 0.1195 mL | 0.239 mL | 0.5975 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
ART558 2603528-97-6 Cell Cycle/Checkpoint DNA Damage/DNA Repair DNA/RNA Synthesis ART 558 ART-558 Inhibitor inhibitor inhibit