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Anti-ST2/IL-1 RL1 Polyclonal Antibody

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Anti-ST2/IL-1 RL1 Polyclonal Antibody
产品编号 TMAB-01776

Anti-ST2/IL-1 RL1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 ST2/IL-1 RL1。Anti-ST2/IL-1 RL1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-ST2/IL-1 RL1 Polyclonal Antibody

Anti-ST2/IL-1 RL1 Polyclonal Antibody

Rating icon 还可以
Anti-ST2/IL-1 RL1 Polyclonal Antibody
产品编号 TMAB-01776

Anti-ST2/IL-1 RL1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 ST2/IL-1 RL1。Anti-ST2/IL-1 RL1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-ST2/IL-1 RL1 Polyclonal Antibody is a Rabbit antibody targeting ST2/IL-1 RL1. Anti-ST2/IL-1 RL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human,Mouse
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (Human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ST2) Polyclonal Antibody, Unconjugated (TMAB-01776) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ST2) Polyclonal Antibody, Unconjugated (TMAB-01776) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample: Kidney (Mouse) Lysate at 40 μg
Primary: Anti-ST2 (TMAB-01776) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
4. Sample:
293t (Human) Cell Lysate at 30 μg
A549 (Human) Cell Lysate at 30 μg
A673 (Human) Cell Lysate at 30 μg
MKN45 (Human) Cell Lysate at 30 μg
Primary: Anti-ST2 (TMAB-01776) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Isoform C: Cell membrane.Isoform B: Secreted. Cell membrane; Single-pass type I membrane protein.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景ST2 is a member of a superfamily containing the Interleukin 1 (IL1) receptor and the Toll-like receptors (TLRs). The TLRs are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. ST2 was originally identified as a protein whose production was stimulated by various proliferation-inducing agents such as PDGF and FGF. More recently, it has been shown to negatively regulate IL1 receptor and Toll-like receptor 4 (TLR4) signaling and to maintain endotoxin tolerance. It has been suggested that the inhibition of TLR4 signaling occurs through the association and sequestering of TLR adaptor molecules such as MyD88 and TIRAP.
抗原信息
免疫原
KLH conjugated synthetic peptide: human ST2 (isoform A,B,C)
抗原种属
Human
基因ID
Uniprot ID
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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