- 全部删除
- 您的购物车当前为空
Anti-ST2/IL-1 RL1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 ST2/IL-1 RL1。Anti-ST2/IL-1 RL1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-ST2/IL-1 RL1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 ST2/IL-1 RL1。Anti-ST2/IL-1 RL1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,975 | 5日内发货 | |
200 μL | ¥ 2,795 | 5日内发货 |
产品描述 | Anti-ST2/IL-1 RL1 Polyclonal Antibody is a Rabbit antibody targeting ST2/IL-1 RL1. Anti-ST2/IL-1 RL1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human,Mouse |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ST2) Polyclonal Antibody, Unconjugated (TMAB-01776) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ST2) Polyclonal Antibody, Unconjugated (TMAB-01776) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Kidney (Mouse) Lysate at 40 μg Primary: Anti-ST2 (TMAB-01776) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kDa Observed band size: 61 kDa 4. Sample: 293t (Human) Cell Lysate at 30 μg A549 (Human) Cell Lysate at 30 μg A673 (Human) Cell Lysate at 30 μg MKN45 (Human) Cell Lysate at 30 μg Primary: Anti-ST2 (TMAB-01776) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kDa Observed band size: 61 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Isoform C: Cell membrane.Isoform B: Secreted. Cell membrane; Single-pass type I membrane protein. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | ST2 is a member of a superfamily containing the Interleukin 1 (IL1) receptor and the Toll-like receptors (TLRs). The TLRs are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. ST2 was originally identified as a protein whose production was stimulated by various proliferation-inducing agents such as PDGF and FGF. More recently, it has been shown to negatively regulate IL1 receptor and Toll-like receptor 4 (TLR4) signaling and to maintain endotoxin tolerance. It has been suggested that the inhibition of TLR4 signaling occurs through the association and sequestering of TLR adaptor molecules such as MyD88 and TIRAP. |
免疫原 | KLH conjugated synthetic peptide: human ST2 (isoform A,B,C) |
抗原种属 | Human |
基因ID | |
Uniprot ID |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
评论内容