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Anti-SMARCA4 Antibody (3I277)

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Anti-SMARCA4 Antibody (3I277)
产品编号 TMAC-00476

Anti-SMARCA4 Antibody (3I277) 是一种 Rabbit 抗体,靶向 SMARCA4。Anti-SMARCA4 Antibody (3I277) 可用于 WB,ICC/IF,IHC,IP。

Anti-SMARCA4 Antibody (3I277)

Anti-SMARCA4 Antibody (3I277)

Rating icon 还可以
Anti-SMARCA4 Antibody (3I277)
产品编号 TMAC-00476

Anti-SMARCA4 Antibody (3I277) 是一种 Rabbit 抗体,靶向 SMARCA4。Anti-SMARCA4 Antibody (3I277) 可用于 WB,ICC/IF,IHC,IP。

规格价格库存数量
50 μL
¥ 1,480
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-SMARCA4 Antibody (3I277) is a Rabbit antibody targeting SMARCA4. Anti-SMARCA4 Antibody (3I277) can be used in WB,ICC/IF,IHC,IP.
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of BRG1 on different lysates using anti-BRG1 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: HepG2.
2. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
6. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
7. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
8. ICC staining BRG1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. ICC staining BRG1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
10. ICC staining BRG1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
11. ICC staining BRG1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
应用
推荐剂量
WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2α) and Brg-1 (also designated SNF2β) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
化学信息
分子量Theoretical: 185 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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