Anti-IL-23 Polyclonal Antibody is a Rabbit antibody targeting IL-23. Anti-IL-23 Polyclonal Antibody can be used in ELISA,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse (predicted:Rat)

1. Tissue/cell: human rhinopolypus tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-IL-23 Polyclonal Antibody, Unconjugated (TMAB-00956) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Mouse Ear tissue lysates
Lane 2: Human A431 cell lysates
Lane 3: Human Jurkat cell lysates
Primary: Anti-IL-23 (TMAB-00956) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: human IL-23

Human

Il23a

Interleukin-23 subunit alpha

Interleukins,Interleukins

Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.