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Anti-HDAC6 Polyclonal Antibody
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产品编号 TMAB-00841
Anti-HDAC6 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 HDAC6。Anti-HDAC6 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-HDAC6 Polyclonal Antibody
Anti-HDAC6 Polyclonal Antibody
还可以产品编号 TMAB-00841
Anti-HDAC6 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 HDAC6。Anti-HDAC6 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-HDAC6 Polyclonal Antibody is a Rabbit antibody targeting HDAC6. Anti-HDAC6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Rat (predicted:Mouse,Cow,Horse,Rabbit) |
| 验证活性 | 1. Sample: HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-HDAC6 (TMAB-00841) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 134 kDa Observed band size: 134 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HDAC6) Polyclonal Antibody, Unconjugated (TMAB-00841) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Nucleus. Cytoplasm. Note=It is mainly cytoplasmic, where it is associated with microtubules. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | HDAC6 is a member of the class II mammalian histone deacetylases. Human HDAC6 is composed of 1215 amino acid residues. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. |
| 免疫原 | KLH conjugated synthetic peptide: human HDAC6 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Acetylation,Class II / Hda1 Class,HDACs |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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