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Anti-CHGA Polyclonal Antibody

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Anti-CHGA Polyclonal Antibody
产品编号 TMAB-00424

Anti-CHGA Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CHGA。Anti-CHGA Polyclonal Antibody 可用于 IHC-P,IHC-Fr,ICC/IF,IF。

Anti-CHGA Polyclonal Antibody

Anti-CHGA Polyclonal Antibody

Rating icon 还可以
Anti-CHGA Polyclonal Antibody
产品编号 TMAB-00424

Anti-CHGA Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CHGA。Anti-CHGA Polyclonal Antibody 可用于 IHC-P,IHC-Fr,ICC/IF,IF。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-CHGA Polyclonal Antibody is a Rabbit antibody targeting CHGA. Anti-CHGA Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG
交叉反应
Human,Rat (predicted:Mouse)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Chromogranin A) Polyclonal Antibody, Unconjugated (TMAB-00424) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Chromogranin A) Polyclonal Antibody, Unconjugated (TMAB-00424) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Chromogranin A) Polyclonal Antibody, Unconjugated (TMAB-00424) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Chromogranin A) polyclonal Antibody, Unconjugated (TMAB-00424) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
应用
IHC-P
IHC-Fr
ICC/IF
IF
推荐剂量
IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle membrane. Secreted. Note=Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq, Jul 2008].
抗原信息
免疫原
KLH conjugated synthetic peptide: human CHGA
抗原种属
Human
基因ID
1113
Uniprot ID
P10645
研究领域
Insulin and insulin-like,Tumor biomarkers,Soma marker,Insulin / Insulin-like,Tumor Associated
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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