Powder: -20°C for 3 years | In solvent: -80°C for 1 year
AM404 is a chemical compound that functions as an inhibitor of endocannabinoid reuptake. It effectively blocks the transport of anandamide, with IC50 values measured in the low micromolar range. Moreover, AM404 exhibits the ability to induce relaxation in rat isolated hepatic arteries contracted with Phenylephrine. This relaxation effect is quantified by a pEC50 value of 7.4 (corresponding to an EC50 of 0.04 μM). Additionally, AM404 possesses a neuroprotective effect.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 437 | 35日内发货 | ||
10 mg | ¥ 787 | 35日内发货 | ||
50 mg | ¥ 3,250 | 35日内发货 | ||
100 mg | ¥ 5,670 | 35日内发货 |
产品描述 | AM404 is a chemical compound that functions as an inhibitor of endocannabinoid reuptake. It effectively blocks the transport of anandamide, with IC50 values measured in the low micromolar range. Moreover, AM404 exhibits the ability to induce relaxation in rat isolated hepatic arteries contracted with Phenylephrine. This relaxation effect is quantified by a pEC50 value of 7.4 (corresponding to an EC50 of 0.04 μM). Additionally, AM404 possesses a neuroprotective effect. |
体外活性 | AM404 reduces C6 glioma cell proliferation with IC 50 values of 4.9 μM. AM404 non-specifically inhibit C6 glioma cell proliferation at concentrations used to block the cellular accumulation of the endocannabinoid anandamide[2]. Cell Viability Assay[2]Cell Line: Rat C6 glioma cells Concentration: 1, 3, 10 and 30 μM Incubation Time: 24, 48, 72 and 96 h Result: Produced a concentration-dependent reduction in cell proliferation that was seen with 24 h of exposure to 10 and 30 μM concentrations and after 48 h at 3 μM. The lowest concentration of AM404 tested, 1 μM, produced a significant, albeit small, reduction in cell proliferation at 72 h. |
体内活性 | AM404 (1-5?mg/kg, i.p.) exerts dose-dependent anxiolytic-like effects in the three models: elevated plus maze, defensive withdrawal and separation-induced ultrasonic vocalizations. Animal Model: Adult male Sprague-Dawley rats (250-300?g)[2]Dosage: 2.5-10?mg/kg Administration: Intraperitoneal (i.p.) Result: Caused a dose-dependent increase in anandamide levels in prefrontal cortex, hippocampus and thalamus. |
分子量 | 395.58 |
分子式 | C26H37NO2 |
CAS No. | 183718-77-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
AM404 183718-77-6 AM 404 AM-404 Inhibitor inhibitor inhibit