Acridine Orange 10-Nonyl Bromide (AO-10NB, 45 μM) 与心磷脂（cardiolipin）结合时，其荧光光谱会发生显著变化：激发波长从 496 nm 蓝移至 450 nm，而发射波长则从 525 nm 红移至 640 nm。研究表明，当向 AO-10NB 溶液中逐步加入含心磷脂（0–30 μM）的薄壁囊泡时，640 nm 处的红色荧光发射强度会随脂质体中心磷脂或其他酸性磷脂的含量增加而发生规律性变化。这种荧光特性的改变可作为检测膜中心磷脂分布的敏感探针。[2]

Acridine Orange 10-Nonyl Bromide is a fluorescent probe for cardiolipin (λex: 489 nm, λem: 525 nm) which can be used to quantify the cardiolipin in isolated mitochondria[1]. when Acridine Orange 10-Nonyl Bromide interacts with cardiolipin, the dye excitation and emission wave lengths shift from 496 and 525 nm to 450 and 640 nm, respectively. Increasing amounts of cardiolipin (0 to 30 μM) and other acidic phospholipids in thin-walled vesicles added to Acridine Orange 10-Nonyl Bromide (45 μM) changes the red fluorescence emission measured at 640 nm according to the liposome composition[2].