Powder: -20°C for 3 years | In solvent: -80°C for 2 years
SF1670 是一种特异性 PTEN 抑制剂,IC50 为 2 μM。
产品描述 | SF1670 is a specific PTEN inhibitor with IC50 of 2 μM. |
靶点活性 | PTEN:2 μM |
体外活性 | SF1670 shows potent cytotoxicity in HBEC, PC-3, H1299 cells with IC50 of 5 μM, 10 μM, 44 μM, respectively. In a mouse aortic ring matrigel angiogenesis model, SF1670 stimulates the angiogenic processes. [1] SF1670 augments chemoattractant-elicited PtdIns(3,4,5)P3 signaling in neutrophils and enhances neutrophil functions. [2] |
体内活性 | Pretreatment of SF1670 (500 nM i.v.) augments bacteria-killing capability in neutropenic mice in both peritonitis and bacterial pneumonia, and decreases the mortality of neutropenia-related pneumonia. [2] |
激酶实验 | PTEN Inhibition Assay: To determine the dose response of potential PTEN inhibitors, doses of test compounds ranging from 1 nM to 250 uM (final reaction mix concentrations) are evaluated in the general PTEN inhibition assay. To obtain performed IC50 data, two separate rounds of the dose response assay are performed. In the first round, PTEN activity is tested in the presence of inhibitor at 10 fold serial dilutions ranging from 1 nM to 250 uM. Once the concentration range is determined, at which PTEN activity changes dramatically, two additional concentration data points within this range are added and the PTEN inhibition assay is then rerun for the second round. The PTEN inhibition IC50 is presented as the inhibitor concentration at which 50% of the PTEN activity. When the assay was run on multiple occasions and gave slightly different IC50 then those are reported as a range of IC50 found. |
细胞实验 | Cells are plated into 96-well plates in RPMI 1640 medium supplemented with 10% FBS and incubated overnight at 37°C. in an incubator containing an atmosphere of 5% CO2. The following day, the medium is replaced and cells are starved by placing in 100 uL of serum-free medium for 3 hours. Serially diluted test compounds are added to the wells and incubated with the cells for 2 hours at 37°C. Compounds are tested in a range from 1 mM to 0.1 nM depending on solubility. MTT is added to the wells at a final concentration of 5 μg/ml and incubated with the cells for 3 more hours. At the end of the incubation, the medium is aspirated and the MTT stain in the cells is dissolved by the addition of 100 μL DMSO. Optical density of each well is then measured at 570 nm using a SpectroMax Plus spectrophotometric plate reader. The IC50 determination from the data was made using Prism software.(Only for Reference) |
别名 | PTP CD45 Inhibitor, PTPase CD45 Inhibitor |
分子量 | 307.34 |
分子式 | C19H17NO3 |
CAS No. | 345630-40-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
DMSO: 30.7 mg/mL (100 mM)
( < 1 mg/mL refers to the product slightly soluble or insoluble )
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
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您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
SF1670 345630-40-2 Autophagy Metabolism PI3K/Akt/mTOR signaling Phosphatase PTEN PTP CD45 Inhibitor inhibit MMAC1 Inhibitor SF 1670 SF-1670 Phosphatase and tensin homolog PTPase CD45 Inhibitor inhibitor